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Effect of additives on the enzymatic hydrolysis of pre-treated wheat straw

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Abstract

Enzymatic hydrolysis of pre-treated wheat straw has been studied. Several additives have been checked as potential improvers of glucose yield in the enzymatic hydrolysis, trying to avoid the cellulases inhibition by the lignin as a cause of the decrease of the saccharification rate of lignocellulosic biomass. Non-ionic surfactants, such as Tween 80 (poly(oxyethylene)20-sorbitan-monooleate) and polyethylene glycol-6000, and proteins, as bovine serum albumin, casein and skimmed milk powder, have been used to improve the sugars yield in the enzymatic hydrolysis. Two pre-treated wheat straw materials, one with high delignification and another with low delignification, have been tested. Three concentrations of each additive have been considered. The results obtained point out that the lignin content of the pre-treated lignocellulosic biomass has a decisive influence in reaching a high yield in the enzymatic saccharification. The additives checked have a moderate effect on the cellulose hydrolysis rate. The more favorable option combines high delignification with the use of a cheap protein such as casein, reaching a glucose yield of 88.6% when the best conditions are employed.

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References

  • Agnihotri S, Johnsen IA, Bøe MS, Øyaas K, Moe S (2015) Ethanol organosolv pretreatment of softwood (Picea abies) and sugarcane bagasse for biofuel and biorefinery applications. Wood Sci Technol 49:881

    Article  CAS  Google Scholar 

  • Akimkulova A, Zhou Y, Zhao X, Liu D (2016) Improving the enzymatic hydrolysis of dilute acid pretreated wheat straw by metal ion blocking of non-productive cellulase adsorption on lignin. Bioresour Technol 208:110–116

    Article  CAS  PubMed  Google Scholar 

  • Bisaria VS, Kondo A (2014) Bioprocessing of renewable resources to commodity bioproducts. Willey, Hoboken

    Book  Google Scholar 

  • Brethauer S, Studer MH, Yang B, Wyman CE (2011) The effect of bovine serum albumin on batch and continuous enzymatic cellulose hydrolysis mixed by stirring or shaking. Bioresour Technol 102:6295–6298

    Article  CAS  PubMed  Google Scholar 

  • Castanon M, Wilke CR (1981) Effects of the surfactant tween 80 on enzymatic hydrolysis of newspaper. Biotechnol Bioeng 23:1365–1372

    Article  CAS  Google Scholar 

  • Chia-wen CH, Cannella D, Jørgensen H, Felby C, Thygesen LG (2015) Cellobiohydrolase and endoglucanase respond differently to surfactants during the hydrolysis of cellulose. Biotechnol Biofuels 8:52

    Article  Google Scholar 

  • CLAL—Italian Dairy Economic Consulting (2019). https://www.clal.it/. Accessed 11 May 2020

  • Desai SG, Converse AO (1997) Substrate reactivity as a function of the extent of reaction in the enzymatic hydrolysis of lignocellulose. Biotechnol Bioeng 56:650–655

    Article  CAS  PubMed  Google Scholar 

  • Drissen RET, Maas RHW, van der Maarel MJEC, Kabel MA, Schols HA, Tamper J, Beeftink HH (2007) A generic model for glucose production from various cellulose sources by a commercial cellulase complex. Biocatal Biotransform 25:419–429

    Article  CAS  Google Scholar 

  • Eriksson T, Börjesson J, Tjerneld F (2002) Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzyme Microb Technol 31:353–364

    Article  CAS  Google Scholar 

  • Gunjikar TP, Sawant SB, Joshi JB (2001) Shear deactivation of cellulase, exoglucanase, endoglucanase, and β-glucosidase in a mechanically agitated reactor. Biotechnol Prog 17:1166–1168

    Article  CAS  PubMed  Google Scholar 

  • Heiss-Blanquet S, Zheng D, Ferreira NL, Lapierre C, Baumberger S (2011) Effect of pretreatment and enzymatic hydrolysis of wheat straw on cell wall composition, hydrophobicity and cellulase adsorption. Bioresour Technol 102:5938–5946

    Article  CAS  PubMed  Google Scholar 

  • Helle SS, Duff SJB, Cooper DG (1993) Effect of surfactants on cellulose hydrolysis. Biotechnol Bioeng 42:611–617

    Article  CAS  PubMed  Google Scholar 

  • Hendriks ATWM, Zeeman G (2009) Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresour Technol 100:10–18

    Article  CAS  PubMed  Google Scholar 

  • Himmel ME, Ding SY, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315:804–807

    Article  CAS  PubMed  Google Scholar 

  • Huijgen WJJ, Smit AT, Reith JH, den Uil H (2011) Catalytic organosolv fractionation of willow wood and wheat straw as pretreatment for enzymatic cellulose hydrolysis. J Chem Technol Biotechnol 86:1428–1438

    Article  CAS  Google Scholar 

  • Huron M, Hudebine D, Ferreira NL, Lachenal D (2016) Impact of delignification on the morphology and the reactivity of steam exploded wheat straw. Ind Crops Prod 79:104–109

    Article  CAS  Google Scholar 

  • Kaar WE, Holtzapple MT (1998) Benefits from Tween during enzymic hydrolysis of corn stover. Biotechnol Bioeng 59:419–427

    Article  CAS  PubMed  Google Scholar 

  • Kim MH, Lee SB, Ryu DDY, Reese ET (1982) Surface deactivation of cellulase and its prevention. Enzyme Microb Technol 4:99–103

    Article  CAS  Google Scholar 

  • Kristensen JB, Börjesson J, Bruun MH, Bruun MH, Tjerneld F, Jørgensen H (2007) Use of surface active additives in enzymatic hydrolysis of wheat straw lignocellulose. Enzyme Microb Technol 40:888–895

    Article  CAS  Google Scholar 

  • Krull S, Eidt L, Hevekerl A, Kuenz A, Prüße U (2017) Itaconic acid production from wheat chaff by Aspergillus terreus. Process Biochem 63:169–176

    Article  CAS  Google Scholar 

  • Kumar R, Wyman CE (2009) Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies. Biotechnol Bioeng 102:1544–1557

    Article  CAS  PubMed  Google Scholar 

  • Lee JM, Shi J, Venditti RA, Jameel H (2009) Autohydrolysis pretreatment of Coastal Bermuda grass for increased enzyme hydrolysis. Bioresour Technol 100:6434–6441

    Article  CAS  PubMed  Google Scholar 

  • Linder M, Mattinen M-L, Kontteli M, Lindeberg G, Ståhlberg J, Drakenberg T, Reinikainen T, Pettersson G, Annila A (1995) Identification of functionally important amino acids in the cellulose-binding domain of Trichoderma reesei cellobiohydrolase I. Protein Sci 4:1056–1064

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu H, Sun J, Leu SY, Chen S (2016) Toward a fundamental understanding of cellulase-lignin interactions in the whole slurry enzymatic saccharification process. Biofuel Bioprod Biorefin 10:648–663

    Article  CAS  Google Scholar 

  • Mahmood M, Al-koofee D (2013) Effect of temperature changes on critical micelle concentration for tween series surfactant. Glob J Sci Front Res Chem 13:1–7

    Google Scholar 

  • Monschein M, Reisinger C, Nidetzky B (2014) Dissecting the effect of chemical additives on the enzymatic hydrolysis of pretreated wheat straw. Bioresour Technol 169:713–722

    Article  CAS  PubMed  Google Scholar 

  • Mooney CA, Mansfield SD, Touhy MG, Saddler JN (1998) The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods. Bioresour Technol 64:113–119

    Article  CAS  Google Scholar 

  • Nakagame S, Chandra RP, Kadla JF, Saddler JN (2010) Enhancing the enzymatic hydrolysis of lignocellulosic biomass by increasing the carboxylic acid content of the associated lignin. Biotechnol Bioeng 108:538–548

    Article  PubMed  Google Scholar 

  • National Renewable Energy Laboratory (2008a) Hames B, Ruiz R, Scarlata C, Sluiter A, Sluiter J and Templeton D. Preparation of Samples for Compositional Analysis. https://www.nrel.gov/docs/gen/fy08/42620.pdf Accessed 11 May 2020

  • National Renewable Energy Laboratory (2008b) Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J and Templeton D. Determination of Sugars, Byproducts, and Degradation Products in Liquid Fraction Process Samples. https://www.nrel.gov/docs/gen/fy08/42623.pdf Accessed 11 May 2020

  • National Renewable Energy Laboratory (2012) Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D and Croker, D. (2012) Determination of Structural Carbohydrates and Lignin in Biomass https://www.nrel.gov/docs/gen/fy13/42618.pdf Accessed 11 May 2020

  • Ooshima H, Sakata M, Harano Y (1986) Enhancement of enzymatic hydrolysis of cellulose by surfactant. Biotechnol Bioeng 28:1727–1734

    Article  CAS  PubMed  Google Scholar 

  • Otzen D (2011) Protein-surfactant interactions: a tale of many states. Biochim Biophys Acta Proteins Proteom 1814:562–591

    Article  CAS  Google Scholar 

  • Ouyang J, Dong Z, Song X, Lee X, Chen M, Yong Q (2010) Improved enzymatic hydrolysis of microcrystalline cellulose (Avicel PH101) by polyethylene glycol addition. Bioresour Technol 101:6685–6691

    Article  CAS  PubMed  Google Scholar 

  • Ouyang J, Li Z, Li X, Ying H, Yong Q (2009) Enhanced enzymatic conversion and glucose production via two-step enzymatic hydrolysis of corncob residue from xylo-oligosaccharides producer’s waste. BioResources 4:1586–1599

    CAS  Google Scholar 

  • Park JW, Takahata Y, Kajiuchi T, Akehata T (1992) Effects of nonionic surfactant on enzymatic hydrolysis of used newspaper. Biotechnol Bioeng 39:117–120

    Article  CAS  PubMed  Google Scholar 

  • Rahikainen J, Mikander S, Marjamaa K, Tamminen T, Lappas A, Viikari L, Kruus K (2011) Inhibition of enzymatic hydrolysis by residual lignins from softwood-study of enzyme binding and inactivation on lignin-rich surface. Biotechnol Bioeng 108:2823–2834

    Article  CAS  PubMed  Google Scholar 

  • Rocha GJM, Silva VFN, Martín C, Adilson R, Gonçalves AR, Nascimento VM, Souto-Maior AM (2013) Effect of xylan and lignin removal by hydrothermal pretreatment on enzymatic conversion of sugarcane bagasse cellulose for second generation ethanol production. Sugar Tech 15:390–398

    Article  CAS  Google Scholar 

  • Rosen MJ, Kunjappu JT (2012) Surfactants and interfacial phenomena, 4th edn. Willey, Hoboken

    Book  Google Scholar 

  • Seo DJ, Fujita H, Sakoda A (2011) Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification. Bioresour Technol 102:9605–9612

    Article  CAS  PubMed  Google Scholar 

  • Sewalt VJH, Glasser WG, Beauchemin KA (1997) Lignin impact on fiber degradation. 3. Reversal of inhibition of enzymatic hydrolysis by chemical modification of lignin and by additives. J Agric Food Chem 45:1823–1828

    Article  CAS  Google Scholar 

  • Vergara P, Wojtusik M, Revilla E, Ladero M, Garcia-Ochoa F, Villar JC (2018a) Wheat straw fractionation by ethanol-water mixture: optimization of operating conditions and comparison with diluted sulfuric acid pre-treatment. Bioresour Technol 256:178–186

    Article  CAS  PubMed  Google Scholar 

  • Vergara P, Ladero M, García-Ochoa F, Villar JC (2018b) Valorization of Cynara cardunculus crops by ethanol-water treatment: optimization of operating conditions. Ind Crops Prod 124:856–862

    Article  CAS  Google Scholar 

  • Vergara P, Ladero M, García-Ochoa F, Villar JC (2018c) Pre-treatment of corn stover, Cynara cardunculus L. stems and wheat straw by ethanol-water and diluted sulfuric acid: comparison under different energy input conditions. Bioresour Technol 270:449–456

    Article  CAS  PubMed  Google Scholar 

  • Wojtusik M, Villar JC, Zurita M, Ladero M, Garcia-Ochoa F (2017) Study of the enzymatic activity inhibition on the saccharification of acid pretreated corn stover. Biomass Bioenergy 98:1–7

    Article  CAS  Google Scholar 

  • Xiang J, Fan JB, Chen N, Chen J, Liang Y (2006) Interaction of cellulase with sodium dodecyl sulfate at critical micelle concentration level. Colloids Surf B Biointerfaces 49:175–180

    Article  CAS  PubMed  Google Scholar 

  • Xiros C, Katapodis P, Christakopoulos P (2009) Evaluation of Fusarium oxysporum cellulolytic system for an efficient hydrolysis of hydrothermally treated wheat straw. Bioresour Technol 100:5362–5365

    Article  CAS  PubMed  Google Scholar 

  • Yang B, Wyman CE (2006) BSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates. J Anat 94:611–617

    CAS  Google Scholar 

  • Yoon SH, Robyt JF (2005) Activation and stabilization of 10 starch-degrading enzymes by Triton X-100, polyethylene glycols, and polyvinyl alcohols. Enzyme Microb Technol 37:556–562

    Article  CAS  Google Scholar 

  • Zhang Q, He G, Wang J, Cai W, Xu Y (2009) Mechanisms of the stimulatory effects of rhamnolipid biosurfactant on rice straw hydrolysis. Appl Energy 86:S233–S237

    Article  CAS  Google Scholar 

  • Zhang Z, Harrison MD, Rackemann DW, Doherty WOS, O’Hara IM (2016) Organosolv pretreatment of plant biomass for enhanced enzymatic saccharification. Green Chem 18:360–381

    Article  Google Scholar 

  • Zheng Y, Pan Z, Zhang R, Wang D, Jenkins B (2008) Non-ionic surfactants and non-catalytic protein treatment on enzymatic hydrolysis of pretreated creeping wild ryegrass. Appl Biochem Biotechnol 146:231–248

    Article  CAS  PubMed  Google Scholar 

  • Zhou Y, Chen H, Qi F, Zhao X, Liu D (2015) Non-ionic surfactants do not consistently improve the enzymatic hydrolysis of pure cellulose. Bioresour technol 182:136–143

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study has been supported by Spanish Ministry of Economy and Competitiveness (MINECO) under contact CTQ2017-84963-C2-2-R, and Madrid Regional Government (Project RETO PROSOST P2013-MAE2907). Grant support for Priscilla Vergara by the National Commission for Scientific and Technological Research (CONICYT- Grant 72160108), of the Ministry of Education of Chile, is also gratefully recognized.

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Authors and Affiliations

  1. Cellulose and Paper Laboratories, INIA, Ctra. de La Coruña km 7.5, 28040, Madrid, Spain

    Priscilla Vergara, José Mª Carbajo & Juan C. Villar

  2. Chemical Engineering and Materials Department, Faculty of Chemistry, UCM, 28040, Madrid, Spain

    Priscilla Vergara, Miguel Ladero & Félix García-Ochoa

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  1. Priscilla Vergara
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  2. Miguel Ladero
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  3. José Mª Carbajo
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  4. Félix García-Ochoa
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  5. Juan C. Villar
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All the authors made substantial contributions to the conception of the work; analysis and interpretation of data and contributed to the writing of this manuscript and approved the final version.

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Correspondence to Juan C. Villar.

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Vergara, P., Ladero, M., Carbajo, J.M. et al. Effect of additives on the enzymatic hydrolysis of pre-treated wheat straw. Braz. J. Chem. Eng. 38, 241–249 (2021). https://doi.org/10.1007/s43153-021-00092-8

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  • DOI: https://doi.org/10.1007/s43153-021-00092-8

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