Abstract

Tritium-labeled lysozyme was used to determine its adsorption on the surface of nanodiamonds produced by detonation synthesis. It was found that the amount of the adsorbed protein depends on the charge of the nanodiamond surface. A multilayer coating is formed on the originally positively charged nanodiamonds, with the adsorption becoming 4–5 times stronger on negatively charged nanodiamonds produced from the original nanodiamonds via their annealing in air. According to IR spectroscopic data, the adsorption of lysozyme results in that the number of loops and turns in the protein structure decreases in the adsorption on two types of nanodiamonds. The use of a tritium probe did not find any significant difference between the structures of protein molecules in their adsorption on nanodiamonds with different functional surface compositions. The orientation of molecules in the surface layer was found to be the same: Protein parts containing amino acid residues of phenylalanine are contacting with the sorbent, and the amino acid residues of proline, which are contained in the loops, are on the surface of the adsorption layer.

中文翻译：

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Pro探针法研究爆轰纳米金刚石表面溶菌酶的吸附层

摘要

t标记的溶菌酶用于确定其在爆轰合成产生的纳米金刚石表面上的吸附。发现吸附的蛋白质的量取决于纳米金刚石表面的电荷。在最初带正电的纳米金刚石上形成多层涂层，在原始纳米金刚石通过在空气中退火后产生的带负电的纳米金刚石上，其吸附作用增强了4-5倍。根据IR光谱数据，溶菌酶的吸附导致在两种类型的纳米金刚石上的吸附中蛋白质结构的环和匝数减少。t探针的使用没有发现蛋白质分子在具有不同功能表面组成的纳米金刚石上的吸附之间的显着差异。