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Tritium-Probe Method in a Study of Adsorption Layers of Lysozyme on the Surface of Detonation Nanodiamonds

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Radiochemistry Aims and scope

Abstract

Tritium-labeled lysozyme was used to determine its adsorption on the surface of nanodiamonds produced by detonation synthesis. It was found that the amount of the adsorbed protein depends on the charge of the nanodiamond surface. A multilayer coating is formed on the originally positively charged nanodiamonds, with the adsorption becoming 4–5 times stronger on negatively charged nanodiamonds produced from the original nanodiamonds via their annealing in air. According to IR spectroscopic data, the adsorption of lysozyme results in that the number of loops and turns in the protein structure decreases in the adsorption on two types of nanodiamonds. The use of a tritium probe did not find any significant difference between the structures of protein molecules in their adsorption on nanodiamonds with different functional surface compositions. The orientation of molecules in the surface layer was found to be the same: Protein parts containing amino acid residues of phenylalanine are contacting with the sorbent, and the amino acid residues of proline, which are contained in the loops, are on the surface of the adsorption layer.

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Funding

The study was financially supported by the Russian Foundation for Basic Research (grant nos. 17-03-00985-a, 18-33-20147-mol-a-ved).

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Authors and Affiliations

  1. Chemical Department, Lomonosov Moscow State University, 119991, Moscow, Russia

    M. G. Chernysheva, G. A. Badun, A. V. Sinolits, A. V. Egorov, T. B. Egorova & A. G. Popov

  2. Belozerskii Institute of Physicochemical Biology, Lomonosov Moscow State University, 119234, Moscow, Russia

    A. L. Ksenofontov

Authors
  1. M. G. Chernysheva
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  2. G. A. Badun
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  3. A. V. Sinolits
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  4. A. V. Egorov
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  5. T. B. Egorova
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  6. A. G. Popov
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  7. A. L. Ksenofontov
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Corresponding author

Correspondence to M. G. Chernysheva.

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Additional information

Translated from Radiokhimiya, No. 2, pp. 185–192, February, 2021 https://doi.org/10.31857/S0033831121020118

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Chernysheva, M.G., Badun, G.A., Sinolits, A.V. et al. Tritium-Probe Method in a Study of Adsorption Layers of Lysozyme on the Surface of Detonation Nanodiamonds. Radiochemistry 63, 227–234 (2021). https://doi.org/10.1134/S1066362221020132

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  • DOI: https://doi.org/10.1134/S1066362221020132

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