Abstract

The current investigation aimed to determine the effects of pre-treatment of acetaminophen on seizure susceptibility and expressions of hippocampal oxidative-nitrosative stress and inflammatory markers after exposure to pentylenetetrazole administration in a mouse model. The seizure symptoms of pentylenetetrazole-treated mice were assessed using Racine classification and latency to first myoclonic jerk. Then, several parameters including TAS, TOS, NO, TNF-α, caspase-3, GABA, and glutamate levels were quantitated by commercial kits. The pre-treatment of acetaminophen significantly suppressed pentylenetetrazole-induced seizures. Also, it significantly increased the TAS and GABA values and significantly decreased the TOS, NO, TNF-α, caspase-3, and glutamate values in the hippocampus. These findings are indicative of the antiseizure potential of pre-treatment with acetaminophen in the pentylenetetrazole-induced acute seizure model of epilepsy. This effect may be due to its impact on antioxidant, anti-inflammatory, and anti-apoptotic pathways.

中文翻译：

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对乙酰氨基酚预处理对PTZ诱发的急性癫痫小鼠模型海马氧化，炎症和凋亡参数的影响

摘要

当前的研究旨在确定对乙酰氨基酚预处理对小鼠模型中戊四氮暴露后癫痫发作的敏感性以及海马氧化亚硝基应激和炎症标志物表达的影响。使用Racine分类和首次肌阵挛性发作的潜伏期评估戊四氮治疗小鼠的癫痫症状。然后，通过商业试剂盒对包括TAS，TOS，NO，TNF-α，caspase-3，GABA和谷氨酸水平在内的几个参数进行定量。对乙酰氨基酚的预处理显着抑制了戊四氮诱导的癫痫发作。而且，它显着增加了海马中的TAS和GABA值，并显着降低了TOS，NO，TNF-α，caspase-3和谷氨酸的值。这些发现表明在戊烯四唑诱导的癫痫急性发作模型中对乙酰氨基酚预处理的抗癫痫潜能。该作用可能是由于其对抗氧化剂，抗炎和抗凋亡途径的影响。