Abstract—We studied the dopamine-, serotonin-, and norepinephrinergic systems in various brain structures of BALB/c male mice on days 15, 42, and 64 of postnatal development (PND) in the model of autism spectrum disorder induced by sodium valproate administration (400 mg/kg, s/c) to pregnant females. It was found that the level of both catechol- and indolamines in the brain structures of control 15-day-old mice is considerably lower than in 64-day-old adult animals. Prenatal administration of sodium valproate (SV) caused a decrease in all parameters of monoaminergic neurotransmission in the striatum of mouse offspring aged 15 days but did not lead to neurochemical changes in other studied brain structures. By PND 42, the general pattern of changes in neurotransmitter concentrations did not differ from the developmental dynamics of neurotransmitter system maturation in the control group. The level of DA kept increasing and by PND 64, did not differ from controls. The parameters of serotonergic system changed similarly, with the peak serotonin concentration by PND 42 and a significant decrease by PND 64, whereas the level of 5-HIAA in the striatum increased gradually with maximum differences observed by PND 64. Thus, the data obtained suggest that administration of SV to pregnant females affects the activity of the dopamine- and serotonergic brain systems in the progeny, inducing its decrease in the striatum by PND 15 followed by recovery to control level by PND 64.

摘要-我们研究了丙戊酸钠诱发的自闭症谱系障碍模型中，在出生后第15天，第42天和第64天，在BALB / c雄性小鼠的各种大脑结构中研究了多巴胺，5-羟色胺和去甲肾上腺素能系统（ 400 mg / kg，s / c）给怀孕的女性。发现在对照的15日龄小鼠的脑结构中儿茶酚和吲哚胺的水平均显着低于64日龄成年动物。产前服用丙戊酸钠（SV）导致15天大的小鼠后代纹状体中单胺能神经传递的所有参数降低，但未导致其他研究的脑部结构发生神经化学变化。由PND 42 对照组中神经递质浓度变化的一般模式与神经递质系统成熟的发育动力学没有差异。DA的水平一直在增加，PND 64与对照组无差异。血清素能系统的参数也有类似的变化，PND 42的血清素浓度达到峰值，PND 64的血清素浓度明显降低，而纹状体中5-HIAA的水平逐渐升高，PND 64观察到的差异最大。因此，获得的数据表明SV对怀孕雌性动物的给药会影响子代中的多巴胺和血清素能神经系统的活动，通过PND 15诱导纹状体减少，然后通过PND 64恢复至对照水平。与控件没有什么不同。血清素能系统的参数也有类似的变化，PND 42的血清素浓度达到峰值，PND 64的血清素浓度明显降低，而纹状体中5-HIAA的水平逐渐升高，PND 64观察到的差异最大。因此，获得的数据表明SV对怀孕雌性动物的给药会影响子代中的多巴胺和血清素能神经系统的活动，通过PND 15诱导纹状体减少，然后通过PND 64恢复至对照水平。与控件没有什么不同。血清素能系统的参数也有类似的变化，PND 42的血清素浓度达到峰值，PND 64的血清素浓度明显降低，而纹状体中5-HIAA的水平逐渐升高，PND 64观察到的差异最大。因此，获得的数据表明SV对怀孕女性的给药会影响子代中的多巴胺和血清素能神经系统的活动，导致PND 15减少纹状体，然后PND 64恢复至对照水平。