Diversity of extremophilic microorganisms in mud volcanoes is largely unexplored. Here, we report the isolation of a novel alkaliphilic, mesophilic, fermentative bacterium (strain F-3ap^T) from a terrestrial mud volcano located at the Taman peninsula, Russia. Cells of strain F-3ap^T are Gram-stain-positive non-motile rods. The formation of endospores is not observed. The temperature range for growth is 14–42 °C, with an optimum at 37 °C. The pH range for growth is 7.5–11.0, with an optimum at pH 9.0. The isolate utilizes various organic polymeric substances, organic acids, carbohydrates, and proteinaceous compounds. The end products of glucose fermentation are ethanol, CO₂, and H₂. The major cellular fatty acids of strain F-3ap^T are C_16:0, C_16:1, and C_14:0. Phylogenetic analysis reveals that strain F-3ap^T belongs to the order Clostridiales, with less than 91% of 16S rRNA gene sequence similarity to any species with a validly published name. The total size of the genome of strain F-3ap^T is 2.98 Mb, and a genomic DNA G + C content is 56.78 mol%. The whole-genome phylogenetic analysis confirms that strain F-3ap^T forms a distinct lineage within Clostridia. We propose to assign strain F-3ap^T to a new species of a novel genus Anaerotalea alkaliphila gen. nov., sp. nov. The type strain is F-3ap^T (= KCTC 15917 ^T = VKM B-3406 ^T).

泥火山中极端微生物的多样性在很大程度上尚未得到开发。在这里，我们报告从位于俄罗斯塔曼半岛的陆地泥火山中分离出一种新型的嗜碱，嗜温，发酵细菌（菌株F-3ap ^T）。F-3ap ^T菌株的细胞是革兰氏染色阳性的非运动杆。没有观察到内生孢子的形成。生长温度范围为14–42°C，最佳温度范围为37°C。生长的pH范围是7.5–11.0，最佳pH值为9.0。该分离物利用了各种有机聚合物，有机酸，碳水化合物和蛋白质化合物。葡萄糖发酵的最终产物是乙醇，CO ₂和H ₂。F-3ap菌株的主要细胞脂肪酸^T是C _16：0，C _16：1和C _14：0。系统发育分析表明，菌株F-3ap ^T属于梭菌，与任何具有有效名称的物种相比，其16S rRNA基因序列相似性不到91％。F-3ap ^T菌株的基因组总大小为2.98 Mb，基因组DNA G + C含量为56.78 mol％。全基因组系统发育分析证实，菌株F-3ap ^T在梭状芽胞杆菌内形成了独特的谱系。我们建议将菌株F-3ap ^T分配给一种新的嗜碱厌氧厌氧杆菌属的新物种。十一月，sp。十一月 类型应变为F-3ap^T（= KCTC 15917  ^T  = VKM B-3406  ^T）。