Objective

This study aimed to explore the expression of lncRNA MALAT1 in patients with T2DM and its clinical significance. A total of 25 normal controls and 69 patients with T2DM were selected.

Methods

Real-time polymerase chain reaction was used to determine the expression level of lncRNA MALAT1 in blood leukocytes of the two groups.

Results

The expression level of lncRNA MALAT1 in patients with T2DM was significantly higher than that in the control group (p < 0.001). The binary regression analysis revealed that lncRNA MALAT1 (p < 0.001, OR = 11.667) and superoxide dismutase (SOD) (p = 0.018, OR = 0.958) were the risk factors for the onset of T2DM. Spearman correlation analysis showed that the expression level of lncRNA MALAT1 correlated positively with cortisol (8 AM), hemoglobin, and blood glucose levels (60, 120, and 180 min) and negatively with SOD and insulin levels after 60 min in the oral glucose tolerance test. The receiver operating characteristic (ROC) results demonstrated that the area under the curve of ROC was 0.804, sensitivity was 78.3%, and specificity was 84% (p < 0.001).

Conclusion

LncRNA MALAT1 was highly expressed in patients with T2DM, and high expression of lncRNA MALAT1 is associated with decreased insulin secretion under hyperglycemic stress.

中文翻译：

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在2型糖尿病患者中，高糖应激下lncRNA MALAT1的高表达与胰岛素分泌减少有关

客观的

本研究旨在探讨lncRNA MALAT1在T2DM患者中的表达及其临床意义。总共选择了25名正常对照和69名T2DM患者。

方法

实时聚合酶链反应用于确定两组患者白细胞中lncRNA MALAT1的表达水平。

结果

T2DM患者中lncRNA MALAT1的表达水平明显高于对照组（p <0.001）。二进制回归分析显示，lncRNA MALAT1（p <0.001，OR = 11.667）和超氧化物歧化酶（SOD）（p = 0.018，OR = 0.958）是T2DM发作的危险因素。Spearman相关分析表明，口服葡萄糖耐量试验中，lncRNA MALAT1的表达水平与皮质醇（上午8点），血红蛋白和血糖水平（60、120和180分钟）呈正相关，与SOD和胰岛素水平呈负相关（60分钟）。测试。接收器工作特性（ROC）结果表明，ROC曲线下的面积为0.804，灵敏度为78.3％，特异性为84％（p <0.001）。

结论

LncRNA MALAT1在T2DM患者中高表达，而lncRNA MALAT1的高表达与高血糖应激下胰岛素分泌减少有关。