Skip to main content

Advertisement

SpringerLink
Log in

High expression of lncRNA MALAT1 is associated with decreased insulin secretion under hyperglycemic stress in patients with type 2 diabetes mellitus

  • Original Article
  • Published:
International Journal of Diabetes in Developing Countries Aims and scope Submit manuscript

Abstract

Objective

This study aimed to explore the expression of lncRNA MALAT1 in patients with T2DM and its clinical significance. A total of 25 normal controls and 69 patients with T2DM were selected.

Methods

Real-time polymerase chain reaction was used to determine the expression level of lncRNA MALAT1 in blood leukocytes of the two groups.

Results

The expression level of lncRNA MALAT1 in patients with T2DM was significantly higher than that in the control group (p < 0.001). The binary regression analysis revealed that lncRNA MALAT1 (p < 0.001, OR = 11.667) and superoxide dismutase (SOD) (p = 0.018, OR = 0.958) were the risk factors for the onset of T2DM. Spearman correlation analysis showed that the expression level of lncRNA MALAT1 correlated positively with cortisol (8 AM), hemoglobin, and blood glucose levels (60, 120, and 180 min) and negatively with SOD and insulin levels after 60 min in the oral glucose tolerance test. The receiver operating characteristic (ROC) results demonstrated that the area under the curve of ROC was 0.804, sensitivity was 78.3%, and specificity was 84% (p < 0.001).

Conclusion

LncRNA MALAT1 was highly expressed in patients with T2DM, and high expression of lncRNA MALAT1 is associated with decreased insulin secretion under hyperglycemic stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. American Diabetes A. Economic costs of diabetes in the U.S. in 2012. Diabetes Care. 2013;36(4):1033–46.

    Article  Google Scholar 

  2. Halim M, Halim A. The effects of inflammation, aging and oxidative stress on the pathogenesis of diabetes mellitus (type 2 diabetes). Diabetes Metab Syndr. 2019;13(2):1165–72.

    Article  PubMed  Google Scholar 

  3. Gomes BF, Accardo CM. Immunoinflammatory mediators in the pathogenesis of diabetes mellitus. Einstein (Sao Paulo). 2019;17(1):eRB4596.

    Article  Google Scholar 

  4. Dong G, Qu L, Gong X, Pang B, Yan W, Wei J. Effect of social factors and the natural environment on the etiology and pathogenesis of diabetes mellitus. Int J Endocrinol. 2019;2019:8749291.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Leung A, Natarajan R. Long noncoding RNAs in diabetes and diabetic complications. Antioxid Redox Signal. 2018;29(11):1064–73.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Feng SD, Yang JH, Yao CH, Yang SS, Zhu ZM, Wu D, et al. Potential regulatory mechanisms of lncRNA in diabetes and its complications. Biochem Cell Biol. 2017;95(3):361–7.

    Article  CAS  PubMed  Google Scholar 

  7. Mei H, Liu Y, Zhou Q, Hu K, Liu Y. Long noncoding RNA MALAT1 acts as a potential biomarker in cancer diagnosis and detection: a meta-analysis. Biomark Med. 2019;13(1):45–54.

    Article  CAS  PubMed  Google Scholar 

  8. Cardamone G, Paraboschi EM, Solda G, Cantoni C, Supino D, Piccio L, et al. Not only cancer: the long non-coding RNA MALAT1 affects the repertoire of alternatively spliced transcripts and circular RNAs in multiple sclerosis. Hum Mol Genet. 2019;28(9):1414–28.

    Article  CAS  PubMed  Google Scholar 

  9. Abdulle LE, Hao JL, Pant OP, Liu XF, Zhou DD, Gao Y, et al. MALAT1 as a diagnostic and therapeutic target in diabetes-related complications: a promising long-noncoding RNA. Int J Med Sci. 2019;16(4):548–55.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Zhang Y, Wu H, Wang F, Ye M, Zhu H, Bu S. Long non-coding RNA MALAT1 expression in patients with gestational diabetes mellitus. Int J Gynaecol Obstet. 2018;140(2):164–9.

    Article  CAS  PubMed  Google Scholar 

  11. Liu SX, Zheng F, Xie KL, Xie MR, Jiang LJ, Cai Y. Exercise reduces insulin resistance in type 2 diabetes mellitus via mediating the lncRNA MALAT1/MicroRNA-382-3p/resistin axis. Mol Ther Nucleic Acids. 2019;18:34–44.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Yan B, Tao Z-F, Li X-M, Zhang H, Yao J, Jiang Q. Aberrant expression of long noncoding RNAs in early diabetic retinopathy. Invest Ophthalmol Vis Sci. 2014;55(2):941.

    Article  CAS  PubMed  Google Scholar 

  13. Zhang M, Gu H, Xu W, Zhou X. Down-regulation of lncRNA MALAT1 reduces cardiomyocyte apoptosis and improves left ventricular function in diabetic rats. Int J Cardiol. 2016;203:214–6.

    Article  PubMed  Google Scholar 

  14. Hu M, Wang R, Li X, Fan M, Lin J, Zhen J, et al. LncRNA MALAT1 is dysregulated in diabetic nephropathy and involved in high glucose-induced podocyte injury via its interplay with beta-catenin. J Cell Mol Med. 2017;21(11):2732–47.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. American Diabetes A. 2. Classification and diagnosis of diabetes: standards of medical care in diabetes-2018. Diabetes Care. 2018;41(Suppl 1):S13–27.

    Article  Google Scholar 

  16. Ogurtsova K, da Rocha Fernandes JD, Huang Y, Linnenkamp U, Guariguata L, Cho NH, et al. IDF Diabetes Atlas: global estimates for the prevalence of diabetes for 2015 and 2040. Diabetes Res Clin Pract. 2017;128:40–50.

    Article  CAS  PubMed  Google Scholar 

  17. Chatterjee S, Khunti K, Davies MJ. Type 2 diabetes. Lancet. 2017;389(10085):2239–51.

    Article  CAS  PubMed  Google Scholar 

  18. Lin C, Yang L. Long noncoding RNA in cancer: wiring signaling circuitry. Trends Cell Biol. 2018;28(4):287–301.

    Article  CAS  PubMed  Google Scholar 

  19. Yao RW, Wang Y, Chen LL. Cellular functions of long noncoding RNAs. Nat Cell Biol. 2019;21(5):542–51.

    Article  CAS  PubMed  Google Scholar 

  20. Zhang N, Geng T, Wang Z, Zhang R, Cao T, Camporez JP, et al. Elevated hepatic expression of H19 long noncoding RNA contributes to diabetic hyperglycemia. JCI Insight. 2018;3(10):e120304.

  21. Ghaedi H, Zare A, Omrani MD, Doustimotlagh AH, Meshkani R, Alipoor S, et al. Genetic variants in long noncoding RNA H19 and MEG3 confer risk of type 2 diabetes in an Iranian population. Gene. 2018;675:265–71.

    Article  CAS  PubMed  Google Scholar 

  22. Zhao X, Rong C, Pan F, Xiang L, Wang X, Hu Y. Expression characteristics of long noncoding RNA uc.322 and its effects on pancreatic islet function. J Cell Biochem. 2018;119(11):9239–48.

    Article  CAS  PubMed  Google Scholar 

  23. Zhang Y, Qu L, Ni H, Wang Y, Li L, Yang X, et al. Expression and function of lncRNA MALAT1 in gestational diabetes mellitus. Adv Clin Exp Med. 2020;29(8):903–10.

    Article  PubMed  Google Scholar 

  24. Shi S, Yang J, Fan W, Zhou Z, Chen G, Zhang J. Effects of LncRNA MALAT1 on microangiopathy and diabetic kidney disease in diabetic rats by regulating ERK/MAPK signaling pathway. Minerva Med. 2020;111(2):184–6.

  25. Liu P, Jia SB, Shi JM, Li WJ, Tang LS, Zhu XH, et al. LncRNA-MALAT1 promotes neovascularization in diabetic retinopathy through regulating miR-125b/VE-cadherin axis. Biosci Rep. 2019;39(5):BSR20181469.

  26. Feng T, Shao F, Wu Q, Zhang X, Xu D, Qian K, et al. miR-124 downregulation leads to breast cancer progression via LncRNA-MALAT1 regulation and CDK4/E2F1 signal activation. Oncotarget. 2016;7(13):16205–16.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Ding H, Wang F, Shi X, Ma H, Du Y, Hou L, et al. LncRNA MALAT1 induces the dysfunction of beta cells via reducing the histone acetylation of the PDX-1 promoter in type 1 diabetes. Exp Mol Pathol. 2020;114:104432.

    Article  CAS  PubMed  Google Scholar 

  28. Gong W, Zhu G, Li J, Yang X. LncRNA MALAT1 promotes the apoptosis and oxidative stress of human lens epithelial cells via p38MAPK pathway in diabetic cataract. Diabetes Res Clin Pract. 2018;144:314–21.

    Article  CAS  PubMed  Google Scholar 

  29. Chen J, Ke S, Zhong L, Wu J, Tseng A, Morpurgo B, et al. Long noncoding RNA MALAT1 regulates generation of reactive oxygen species and the insulin responses in male mice. Biochem Pharmacol. 2018;152:94–103.

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This study was supported by the Natural Science Foundation of China (No. 81960875).

Author information

Author notes

  1. Da-wei Yang and Lianji Zhou contributed equally to this work.

Authors and Affiliations

  1. JiNan University, Guangzhou, China

    Da-wei Yang & Biao-liang Wu

  2. Affiliated Hospital of Youjiang Medical University For Nationalities, Zhongshan No 2 Road 18, Baise, 533000, China

    Da-wei Yang, Lianji Zhou, Qing Huang, Linan Ou & Biao-liang Wu

Authors
  1. Da-wei Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lianji Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qing Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Linan Ou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Biao-liang Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Biao-liang Wu.

Ethics declarations

Ethics approval

All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and national research committee and with the World Medical Association Declaration of Helsinki or comparable ethical standards. The study was approved by the ethics committee of the hospital, and all participants signed an informed consent form.

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, Dw., Zhou, L., Huang, Q. et al. High expression of lncRNA MALAT1 is associated with decreased insulin secretion under hyperglycemic stress in patients with type 2 diabetes mellitus. Int J Diabetes Dev Ctries 42, 70–76 (2022). https://doi.org/10.1007/s13410-021-00945-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13410-021-00945-5

Keywords

Search

Navigation