In ecotoxicology, in vitro testing on cell cultures represents an ideal alternative to in vivo strategies for emerging contaminants. These tests have limited use particularly with marine invertebrates like the clams Ruditapes decussatus. In the present study, a primary culture of R. decussatus haemocytes was realized for the first time in order to determine the effect of metals (copper, zinc, and cobalt) on haemocyte parameters like viability and phagocytosis. Results showed that (i) among the studied medium, the modified Leibovitz (L-15) is the best for R. decussatus haemocytes primary culture. (ii) The primary culture system used here represents a suitable in vitro model for assessing cytotoxic responses, (iii) a decrease of cell viability and phagocytosis after 24 h exposure to 100 μg mL⁻¹ CoSO₄ and an increase of phagocytosis after 24 h exposure to 50 μg mL⁻¹CuSO₄.

在生态毒理学中，细胞培养物的体外测试代表了新出现污染物的体内策略的理想替代方案。这些测试的用途有限，特别是对海生无脊椎动物，如蛤蜊Ruditapes decussatus。在本研究中，首次实现了红豆杉血细胞的原代培养，以确定金属（铜、锌和钴）对血细胞参数（如活力和吞噬作用）的影响。结果表明，(i) 在研究的培养基中，改良的 Leibovitz (L-15) 是R. decussatus最好的血细胞原代培养。(ii) 此处使用的原代培养系统代表用于评估细胞毒性反应的合适体外模型，(iii) 暴露于 100 μg mL ^-1 CoSO ₄ 24 小时后细胞活力和吞噬作用降低，24 小时后吞噬作用增加暴露于 50 μg mL ^-1 CuSO ₄。