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Establishment of primary cell culture of Ruditapes decussatus haemocytes for metal toxicity assessment

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Abstract

In ecotoxicology, in vitro testing on cell cultures represents an ideal alternative to in vivo strategies for emerging contaminants. These tests have limited use particularly with marine invertebrates like the clams Ruditapes decussatus. In the present study, a primary culture of R. decussatus haemocytes was realized for the first time in order to determine the effect of metals (copper, zinc, and cobalt) on haemocyte parameters like viability and phagocytosis. Results showed that (i) among the studied medium, the modified Leibovitz (L-15) is the best for R. decussatus haemocytes primary culture. (ii) The primary culture system used here represents a suitable in vitro model for assessing cytotoxic responses, (iii) a decrease of cell viability and phagocytosis after 24 h exposure to 100 μg mL−1 CoSO4 and an increase of phagocytosis after 24 h exposure to 50 μg mL−1CuSO4.

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Acknowledgements

The authors thank Dr Nihel Ammous for her helpful technical assistance.

Funding

This work was supported by the Ministry of higher education and scientific research, University of Sfax, Tunisia.

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Authors and Affiliations

  1. Center of Biotechnology of Sfax, Laboratory: Molecular Biotechnology of Eukaryotes, University of Sfax, Sfax, Tunisia

    Rim Ladhar-Chaabouni, Wajdi Ayadi, Emna Sahli & Raja Mokdad-Gargouri

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  1. Rim Ladhar-Chaabouni
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  2. Wajdi Ayadi
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  3. Emna Sahli
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  4. Raja Mokdad-Gargouri
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Correspondence to Rim Ladhar-Chaabouni.

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Editor: Tetsuji Okamoto

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Ladhar-Chaabouni, R., Ayadi, W., Sahli, E. et al. Establishment of primary cell culture of Ruditapes decussatus haemocytes for metal toxicity assessment. In Vitro Cell.Dev.Biol.-Animal 57, 477–484 (2021). https://doi.org/10.1007/s11626-021-00561-x

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