Myocardial infarction (MI) is a serious threat to human health that creates millions of death annually. In this study, we went on to examine molecular mechanism of lavender oil that which by give rises to cardioprotection against MI. An MI model was established in male Wistar rats by ligation of the left anterior descending coronary artery (LAD) and lavender oil at 100 mg/kg concentration 1 h after reperfusion was intraperitoneally administrated. ELISA assay was used to evaluate the activities of myocardial injury markers. Western blot and immunohistochemical assays were used to evaluate the expression of beclin-1, LC3II/LC3I ratio, CD34 and vascular endothelial growth factor (VEGF). RT-PCR was applied to investigate the mRNA levels of apoptotic factors. Compared to sham, increased levels of myocardial injury markers, pro-apoptotic and autophagic factors were found in MI rats that were reversed by post-treatment with lavender oil. Likewise, reduced levels of anti-apoptotic factors and VEGF were observed in MI/R group compared to sham that reversed by lavender oil. Collectively, our findings showed the validity of lavender oil as an excellent candidate to create cardioprotection effects against MI/R injury.

心肌梗塞（MI）是对人类健康的严重威胁，每年造成数百万人死亡。在这项研究中，我们继续研究了薰衣草油的分子机理，该分子机理可提高心肌对MI的保护作用。腹腔注射再灌注1 h后，结扎左冠状动脉前降支（LAD）和浓度为100 mg / kg的薰衣草油，在雄性Wistar大鼠中建立MI模型。ELISA法用于评估心肌损伤标志物的活性。使用蛋白质印迹和免疫组化方法评估beclin-1，LC3II / LC3I比，CD34和血管内皮生长因子（VEGF）的表达。RT-PCR被用于研究凋亡因子的mRNA水平。与假手术相比，心肌损伤标记物水平增加，在通过熏衣草油后处理逆转的MI大鼠中发现促凋亡和自噬因子。同样，与薰衣草油逆转的假手术相比，MI / R组的抗凋亡因子和VEGF的水平降低。总的来说，我们的研究结果表明薰衣草油是产生抗MI / R损伤心脏保护作用的优秀候选者的有效性。