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Lavender Oil Attenuates Myocardial Ischemia/Reperfusion Injury Through Inhibition of Autophagy and Stimulation of Angiogenesis

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Abstract

Myocardial infarction (MI) is a serious threat to human health that creates millions of death annually. In this study, we went on to examine molecular mechanism of lavender oil that which by give rises to cardioprotection against MI. An MI model was established in male Wistar rats by ligation of the left anterior descending coronary artery (LAD) and lavender oil at 100 mg/kg concentration 1 h after reperfusion was intraperitoneally administrated. ELISA assay was used to evaluate the activities of myocardial injury markers. Western blot and immunohistochemical assays were used to evaluate the expression of beclin-1, LC3II/LC3I ratio, CD34 and vascular endothelial growth factor (VEGF). RT-PCR was applied to investigate the mRNA levels of apoptotic factors. Compared to sham, increased levels of myocardial injury markers, pro-apoptotic and autophagic factors were found in MI rats that were reversed by post-treatment with lavender oil. Likewise, reduced levels of anti-apoptotic factors and VEGF were observed in MI/R group compared to sham that reversed by lavender oil. Collectively, our findings showed the validity of lavender oil as an excellent candidate to create cardioprotection effects against MI/R injury.

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The authors confirm that the data supporting the findings of this study are available within the article [and/or] its supplementary materials.

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Acknowledgements

Present work was funded by a research grant from Physiology Research Center in Iran University of Medical Sciences (Ethical number :IR.IUMS.REC 1396.31395).

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Author notes

  1. Maryam Naseroleslami and Kamran Rakhshan have contributed equally to this work.

Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Maryam Naseroleslami

  2. Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Kamran Rakhshan

  3. Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran

    Nahid Aboutaleb

  4. Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Nahid Aboutaleb

  5. Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Faramarz Souri

Authors
  1. Maryam Naseroleslami
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  2. Kamran Rakhshan
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  3. Nahid Aboutaleb
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  4. Faramarz Souri
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Contributions

Conceptualization and Study design, N.A.; investigation and data collection, M.N., K.R., and F.S; writing—original draft preparation, K.R.; writing—review and editing, M.N and N.A. The animal use, experimental procedures and care protocols were approved by School of Medicine Animal Care and Use Committee of Iran University of Medical Sciences. All protocols and animal operation rules were confirmed by the Animal Ethical Committee of Iran University of Medical Sciences.

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Correspondence to Nahid Aboutaleb.

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Naseroleslami, M., Rakhshan, K., Aboutaleb, N. et al. Lavender Oil Attenuates Myocardial Ischemia/Reperfusion Injury Through Inhibition of Autophagy and Stimulation of Angiogenesis. Iran J Sci Technol Trans Sci 45, 1201–1209 (2021). https://doi.org/10.1007/s40995-021-01123-2

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  • DOI: https://doi.org/10.1007/s40995-021-01123-2

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