Vinasse sugarcane is a valuable byproduct of the ethanol production process, presenting a perspective of volume increase with the development of second generation ethanol (2GE). However, this byproduct needs new methods of treatment and management for sustainability. Besides that, 2GE vinasse can be associated with some compounds (such as furan derivatives, phenolic compounds and organic acids), depending on the process used to solubilize hemicellulose, which could compromise vinasse destination or utilization. For this reason, detoxification methods of the hemicellulosic hydrolysates, from which vinasse is obtained in subsequent steps, are crucial. This study aimed to investigate whether the biological detoxification of vinasse from 2GE presents a difference concerning the microbial activity of biodegradation and toxicity when compared to vinasse without the detoxification process. Two vinasses (1, before; and 2, after detoxification) from fermented sugarcane bagasse (hemicellulose fraction) acid hydrolysate (supplemented with its molasses), under different concentrations: 2.5; 5 e 10% were evaluated. Their physicochemical characterization, biodegradation microbial activity (through Bartha and Pramer respirometric method, with total count of heterotrophic bacteria and fungi), and toxicity evaluation (through bioassays with Lactuca sativa at concentraction: 2.5; 5 e 10% and Daphnia similis to 1.5; 2.5; 3.5; 4.5; 5 and 10%) were performed. The results indicated high mineral and organic matter, which under a specific circumstance (2.5% of soil conditioning), enabled high efficiency in biodegradation (>80%). The bioassays with L. sativa signaled negative effect for radicular growth when the vinasses were applied at a concentration of 5 and 10% (sublethal effect and delayed root growth). Acute effects were observed in D. similis, with 50% of immobilization, at concentrations of 4.13% and 4.74% for vinasses 1 and 2, respectively. These results indicate that the biodegradation of vinasse from sugarcane bagasse acid hydrolysate occurs at relatively low levels (up to 5%) and suggests that higher concentrations (≥10%) may impair the growth of soil-associated microorganisms.

Vinasse甘蔗是乙醇生产过程中的宝贵副产品，随着第二代乙醇（2GE）的发展，其产量有望增加。但是，这种副产品需要新的治疗和管理方法以实现可持续性。除此之外，取决于溶解半纤维素的过程，2GE酒糟可以与某些化合物（例如呋喃衍生物，酚类化合物和有机酸）结合，这可能会损害酒糟的目的地或利用。因此，至关重要的是半纤维素水解产物的解毒方法，在后续步骤中可从中获得酒糟。这项研究旨在调查从2GE提取的酒糟的生物解毒与未进行解毒过程的酒糟相比，在生物降解和毒性的微生物活性方面是否存在差异。来自发酵甘蔗渣（半纤维素级分）酸水解产物（补充糖蜜）的两个酒糟（分别为解毒前后的1个和2个），浓度不同：2.5；评估5 e 10％。它们的理化特性，生物降解微生物活性（通过Bartha和Pramer呼吸测定法，以及异养细菌和真菌的总数）和毒性评估（通过生物测定 从解毒后的发酵甘蔗渣（半纤维素馏分）酸水解产物（补充其糖蜜）中，浓度不同：2.5；评估5 e 10％。它们的理化特性，生物降解微生物活性（通过Bartha和Pramer呼吸测定法，以及异养细菌和真菌的总数）和毒性评估（通过生物测定 从解毒后的发酵甘蔗渣（半纤维素馏分）酸水解产物（补充其糖蜜）中，浓度不同：2.5；评估5 e 10％。它们的理化特性，生物降解微生物活性（通过Bartha和Pramer呼吸测定法，以及异养细菌和真菌的总数）和毒性评估（通过生物测定紫花苜蓿的结节数：2.5；5 e 10％，水蚤（Daphnia similis）为1.5；2.5; 3.5; 4.5; 进行5％和10％）。结果表明，高矿物质和有机物在特定情况下（土壤调节的2.5％）能够实现高效率的生物降解（> 80％）。当酒糟的浓度为5％和10％时，具有紫花苜蓿的生物测定表明其对根系生长具有负面影响（亚致死作用和根系生长延迟）。在D. similis中观察到急性作用，固定率为50％，酒窖1和2的浓度分别为4.13％和4.74％。这些结果表明，甘蔗渣酸水解液中酒糟的生物降解发生在相对较低的水平（最高5％），并且表明较高的浓度（≥10％）可能会损害土壤相关微生物的生长。