The growing impact of organic and inorganic pollutants on the environment has led to the development of numerous strategies to counteract this effect. Among all, green technologies such as phytoremediation, has won relevance for its low cost as well as being an eco-friendly and sustainable process. In the past years, tobacco hairy roots (HRs) have become a good example of the effectiveness of plants as a tool for removing different pollutants. In this sense, the understanding of the intracellular mechanisms involved in the phytoremediation process has become of great interest. Glycerophospholipids (GLPs) are a key factor on intracellular signaling, given that they can activate different pathways, despite there is not enough information available about their levels and fluctuation under certain stress conditions. The aim of this work was to develop of a new chromatographic method coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) with selected reaction monitoring (SRM) and data dependent scan detection for the evaluation of GLP profile after the exposure to phenol, to get a better understanding of lipids turnover and their effect on signaling pathways. This highly sensitive method allows the identification, separation and quantification in less than a 20 min-run of the eleven most relevant GPL species present in hairy tobacco root extracts after phenol exposure, and is suitable for evaluating small changes in GPL levels.

有机和无机污染物对环境的影响越来越大，导致人们开发了许多策略来抵消这种影响。其中，诸如植物修复之类的绿色技术因其低成本以及生态友好和可持续的过程而赢得了关注。在过去几年中，烟草毛状根（HRs）已成为植物作为去除不同污染物的工具的有效性的一个很好的例子。从这个意义上讲，对涉及植物修复过程的细胞内机制的理解已经引起了极大的兴趣。甘油磷脂（GLP）是细胞内信号传导的关键因素，尽管在某些压力条件下，尽管它们的水平和起伏不多，但它们可以激活不同的途径。这项工作的目的是开发一种新的色谱方法，结合电喷雾电离串联质谱（LC-ESI-MS / MS）和选定的反应监测（SRM）和数据相关的扫描检测，以评估暴露后的GLP谱图苯酚，以更好地了解脂质周转及其对信号通路的影响。这种高度灵敏的方法可以在少于20分钟的时间内对毛发烟草根提取物中存在的11种最相关的GPL物种进行鉴定，分离和定量分析，而且这些酚均适用于评估GPL含量的微小变化。