Abstract—

From a technogenic soda sludge storage, 58 cultures of alkalitolerant bacteria with amylase, lipase, protease, and cellulase activities were isolated and identified using a medium with selective substrates in the absence of extreme conditions (pH 8) and a rich medium with pH 11 without selective substrates. The effect of pH and NaCl concentration on the growth and hydrolytic activity of Pseudomonas peli, Paenarthrobacter nitroquajacolicus, and Microbacterium kitamiense with lipase and amylase activities was studied. Amylases of the cultures isolated on media with pH 11 and 8 were shown to exhibit the highest activity at pH 10 and 6, respectively, while the specific activity of extracellular lipase of P. peli isolated at pH 8 reached its maximum at pH 11. On the medium with pH 11, Bacillus aequororis, Brevibacterium pityocampae, Microbacterium kitamiense, Microcella putealis, Oerskovia paurometabola, O. enterophila, and O. jenensis with alkaline amylase activity were isolated.

中文翻译：

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苏打污泥贮存中具有水解活性的耐碱性细菌的分离与鉴定

摘要—

从技术纯碱污泥存储中，分离出58种具有淀粉酶，脂肪酶，蛋白酶和纤维素酶活性的耐碱细菌培养物，并使用在没有极端条件（pH 8）的情况下使用带有选择性底物的培养基和在pH 11的富集培养基（没有选择性底物。研究了pH和NaCl浓度对假单胞菌，硝基拟杆菌和北小生菌具有脂肪酶和淀粉酶活性的生长和水解活性的影响。在pH为11和8的培养基上分离的培养物的淀粉酶分别在pH 10和6时表现出最高的活性，而P. peli的细胞外脂肪酶的比活性。在pH值为11的条件下分离到的酸性菌在pH值为11的条件下达到最大值。在碱性pH值为11的培养基中，分离出了碱性淀粉酶活性的水母芽孢杆菌，海藻短杆菌，kitamiense微小杆菌，腐臭小，菌，Oerskovia paurometabola，O。enterophila和O. jenensis。