Skip to main content
SpringerLink
Log in

Isolation and Identification of Alkalitolerant Bacteria with Hydrolytic Activity from a Soda Sludge Storage

  • EXPERIMENTAL ARTICLES
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract—

From a technogenic soda sludge storage, 58 cultures of alkalitolerant bacteria with amylase, lipase, protease, and cellulase activities were isolated and identified using a medium with selective substrates in the absence of extreme conditions (pH 8) and a rich medium with pH 11 without selective substrates. The effect of pH and NaCl concentration on the growth and hydrolytic activity of Pseudomonas peli, Paenarthrobacter nitroquajacolicus, and Microbacterium kitamiense with lipase and amylase activities was studied. Amylases of the cultures isolated on media with pH 11 and 8 were shown to exhibit the highest activity at pH 10 and 6, respectively, while the specific activity of extracellular lipase of P. peli isolated at pH 8 reached its maximum at pH 11. On the medium with pH 11, Bacillus aequororis, Brevibacterium pityocampae, Microbacterium kitamiense, Microcella putealis, Oerskovia paurometabola, O. enterophila, and O. jenensis with alkaline amylase activity were isolated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Ali, S.S., Habib, I., and Riaz, T., Screening and characterization of alkaliphilic bacteria from industrial effluents, Punjab Univ. J. Zool., 2009, vol. 24, pp. 49–60.

    Google Scholar 

  2. Anish, R., Rahman, M.S., and Rao, M., Application of cellulases from an alkalothermophilic Thermomonospora sp. in biopolishing of denims, Biotechnol. Bioeng., 2007, vol. 96, pp. 48–56.

    Article  CAS  Google Scholar 

  3. Atlas, R.M., Handbook of Microbiological Media, Parks, L.C., Ed., Boca Raton: CRC press, 1993.

    Google Scholar 

  4. Bezborodov, A.M. and Zagustina, N.A., Lipases in catalytic reactions of organic chemistry, Appl. Biochem. Microbiol., 2014, vol. 50, pp. 313–337.

    Article  CAS  Google Scholar 

  5. Blinov, S.M., Maksimovich, N.G., Naidanova, N.F., Shlykov, V.G., and Potapov, S.S., Mineralogical basis of waste treatment in the Berezniki Soda Plant, Mineralogiya Tekhnogeneza, 2003, vol. 4, pp. 51–55.

    Google Scholar 

  6. Borzenko, S.V. and Zamana, L.V., Sulfate reduction as a factor of soda water formation in Lake Doroninskoe (Eastern Transbaikalia), Vestn. Tomsk State Univ., 2008, no. 312, pp. 188–193.

  7. Febriani Rayyana, Ulya, M., Oesman, F., Akhmaloka, and Iqbalsyah, T.M., Low molecular weight alkaline thermostable α-amylase from Geobacillus sp. nov., Heliyon, 2019, vol. 5, e02171.

    Article  CAS  Google Scholar 

  8. Grant, W.D. and Sorokin, D.Yu., Distribution and diversity of soda lake alkaliphiles, in Extremophiles Handbook, Horikoshi, K., Antranikian, G., Bull, A., Robb, F., and Stetler, K., Eds., Tokyo: Springer-Verlag, 2011, pp. 27–54.

    Google Scholar 

  9. Gupta, R., Beg, Q.K., and Lorenz, P., Bacterial alkaline proteases: molecular approaches and industrial applications, Appl. Microbiol. Biotechnol., 2002, vol. 59, pp. 15–32.

    Article  CAS  Google Scholar 

  10. Hasan, F., Shah, A.A., Javed, S., and Hameed, A., Enzymes used in detergents: lipases, Afr. J. Biotechnol., 2010, vol. 9, pp. 4836–4844.

    CAS  Google Scholar 

  11. Horikoshi, K., Alkaliphiles: some applications of their products for biotechnology, Microbiol. Mol. Biol. Rev., 1999, vol. 63, pp. 735–750.

    Article  CAS  Google Scholar 

  12. Jagtap, S. and Rao, M., Purification and properties of a low molecular weight 1,4-β-D-glucan glucohydrolase having one active site for carboxymethyl cellulose and xylan from an alkalothermophilic Thermomonospora sp., Biochem. Biophys. Res. Commun., 2005, vol. 329, pp. 111–116.

    Article  CAS  Google Scholar 

  13. Kalwasińska, A., Felföldi, T., Szab, A.J., Deja-Sikora, E., Kosobucki, P., and Walczak, M., Microbial communities associated with the anthropogenic, highly alkaline environment of a saline soda lime, Antonie van Leeuwenhoek, 2017, vol. 110, pp. 945–962.

    Article  Google Scholar 

  14. Kevbrin, V.V., Isolation and cultivation of alkaliphiles, in Alkaliphiles in Biotechnology, Mamo, G. and Mattiasson, B., Eds., Cham: Springer, 2019, Advances in Biochemical Engineering/Biotechnology, vol. 172, pp. 53–84.

  15. Kubrak, O.I., Storey, J.M., Storey, K.B., and Lushchak, V.I., Production and properties of α-amylase from Bacillus sp. BKL20, Can. J. Microbiol., 2010, vol. 56, pp. 279–288.

    Article  CAS  Google Scholar 

  16. Margesin, R., Feller, G., Hämmerle, M., Stegner, U., and Schinner, F., A colorimetric method for the determination of lipase activity in soil, Biotechnol. Lett., 2002, vol. 24, pp. 27–33.

    Article  CAS  Google Scholar 

  17. Mc Tigue, M.A., Kelly, C.T., Doyle, E.M., and Fogarty, W.M., The alkaline amylase of the alkalophilic Bacillus sp. IMD 370, Enzyme Microb. Technol., 1995, vol. 17, pp. 570–573.

    Article  CAS  Google Scholar 

  18. Novozhilov, E.V. and Poshina, D.N., Biotechnologies in cellulose production for chemical treatment (a review), Kh-imiya rastitel’nogo syr’ya, 2011, no. 3, pp. 15–32.

  19. Oren, A., Industrial and environmental applications of halophilic microorganisms, Environ. Technol., 2010, vol. 31, pp. 825–834.

    Article  CAS  Google Scholar 

  20. Randagurueva, A.A. and Lavrent’eva, E.V., Extracellular protease activity in natural samples of thermal springs of the Baikal region, Izv. Irkutsk State Univ., Ser. Nauki o Zemle, 2009, vol. 2, no. 2, pp. 162–166.

  21. Ren, L., Han, Y., Yang, S., Tan, X., Wang, J., Zhao, X., Fan, J., Dong, T., and Zhou, Z., Isolation, identification and primary application of bacteria from putrid alkaline silica sol, Front. Chem. Sci. Eng., 2014, vol. 8, pp. 330–339.

    Article  CAS  Google Scholar 

  22. Rios, N.S., Pinheiro, B.B., Pinheiro, M.P., Bezerra, R.M., Sousa dos Santos, J.C., and Gonçalves, L.R.B., Biotechnological potential of lipases from Pseudomonas: Sources, properties and applications, Process Biochem., 2018, vol. 75, pp. 99–120.

    Article  CAS  Google Scholar 

  23. Roadcap, G.S., Sanford, R.A., Jin, Q., Pardinas, J.R., and Bethke, C.M., Extremely alkaline (pH > 12) ground water hosts diverse microbial community, Ground Water, 2006, vol. 44, pp. 511–517.

    Article  CAS  Google Scholar 

  24. Sarethy, I.P., Saxena, Y., Kapoor, A., Sharma, M., Sharma, S.K., Gupta, V., and Gupta, S., Alkaliphilic bacteria: applications in industrial biotechnology, J. Ind. Microbiol. Biotechnol., 2011, vol. 38, pp. 769–790.

    Article  CAS  Google Scholar 

  25. Saxena, R.K., Dutt, K., Agarwal, L., and Nayyar, P., A highly thermostable and alkaline amylase from a Bacillus sp. PN5, Biores. Technol., 2007, vol. 98, pp. 260–265.

    Article  CAS  Google Scholar 

  26. Sharma, K.M., Kumar, R., Panwar, S., and Kuma, A., Microbial alkaline proteases: optimization of production parameters and their properties, J. Genet. Eng. Biotechnol., 2017, vol. 15, pp. 115–126.

    Article  Google Scholar 

  27. Shilova, A.V., Maksimov, A.Yu., and Maksimova, Yu.G., Metagenomic analysis of the genus-level composition of bacterial communities of the soil, water, and sediments of the new and old sludge storage of the OAO Soda (Berezniki, Perm krai), Biomika, 2018, vol. 10, no. 1, pp. 24–27.

    Google Scholar 

  28. Shilova, A.V., Maksimov, A.Yu., and Maksimova, Yu.G., Microbiome changes as a marker of environmental remediation in the soda sludge storage of the Berezniki Soda Plant, Voda Ekologiya: Problemy Resheniya, 2020, no. 1(81), pp. 81–94.

  29. Sorokin, D.Y., Banciu, H.L., and Muyzer, G., Functional microbiology of soda lakes, Curr. Opin. Microbiol., 2015, vol. 25, pp. 88–96.

    Article  CAS  Google Scholar 

  30. Zavarzin, G.A. and Zhilina, T.N., Soda lakes: a natural model of the ancient biosphere of the continent, Priroda, 2000, no. 2, pp. 45–55.

Download references

Funding

This study was supported by the Russian Foundation for Basic Research, project no. 19-34-90103.

Author information

Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, 614081, Perm, Russia

    A. V. Shilova, A. Yu. Maksimov & Yu. G. Maksimova

  2. Perm State National Research University, 614990, Perm, Russia

    A. Yu. Maksimov & Yu. G. Maksimova

Authors
  1. A. V. Shilova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Yu. Maksimov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu. G. Maksimova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Shilova.

Ethics declarations

Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by A. Panyushkina

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shilova, A.V., Maksimov, A.Y. & Maksimova, Y.G. Isolation and Identification of Alkalitolerant Bacteria with Hydrolytic Activity from a Soda Sludge Storage. Microbiology 90, 166–175 (2021). https://doi.org/10.1134/S0026261721020120

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261721020120

Keywords:

Search

Navigation