SummaryThis study aimed to describe the viability of domestic feline spermatozoa after epididymal tail vitrification. For this, 10 pairs of testis–epididymis complexes were used. The epididymal tails were vitrified using the solid-surface vitrification (SSV) method, in which two vitrification media containing ethylene glycol (EG) 40% or glycerol (GLY) 40% were tested. Vitrification with the presence of EG resulted in better results for all sperm motility parameters (motility, vigour and CASA) compared with GLY (P < 0.05). There were no statistical differences for sperm viability and acrosome integrity, plasma membrane integrity, or overall health of morphologically normal sperm before or after vitrification among experimental groups. In conclusion, epididymal tail vitrification appears to be a suitable method for long-term storage of cat sperm, especially if the procedure is performed with EG as the cryoprotectant.

中文翻译：

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附睾尾部固体表面玻璃化作为家猫精子冷冻库的有效方法

摘要本研究旨在描述家猫精子在附睾尾部玻璃化后的生存能力。为此，使用了 10 对睾丸-附睾复合物。附睾尾部使用固体表面玻璃化 (SSV) 方法进行玻璃化，其中测试了两种含有 40% 乙二醇 (EG) 或 40% 甘油 (GLY) 的玻璃化介质。与 GLY 相比，存在 EG 的玻璃化冷冻在所有精子活力参数（运动性、活力和 CASA）方面产生了更好的结果。磷< 0.05)。实验组之间在玻璃化之前或之后，精子活力和顶体完整性、质膜完整性或形态正常精子的整体健康没有统计学差异。总之，附睾尾部玻璃化似乎是长期储存猫精子的合适方法，尤其是在使用 EG 作为冷冻保护剂的情况下。