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Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Published online by Cambridge University Press:  08 April 2021

Silmara L.G. Lima ,
Airton R.B. Soares ,
Leanne Stalker ,
Regiane R. Santos Open the ORCID record for Regiane R. Santos [Opens in a new window]  and
Sheyla F.S. Domingues Open the ORCID record for Sheyla F.S. Domingues [Opens in a new window]
Silmara L.G. Lima
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil Postgraduate Program in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, Pará, Brazil
Airton R.B. Soares
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Leanne Stalker
Affiliation:
Department of Biomedical Science, University of Guelph, Guelph, Ontario, Canada
Regiane R. Santos*
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Sheyla F.S. Domingues
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil Postgraduate Program in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, Pará, Brazil Postgraduate Program of Animal Reproduction in Amazon, Institute of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
*
Author for correspondence: Regiane R. Santos. Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, BR 316 Km 61, CEP 68740–970, Castanhal, Pará, Brazil. Tel: +55 91 33114707. E-mail: regiane.r.dos.santos@gmail.com
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Summary

This study aimed to describe the viability of domestic feline spermatozoa after epididymal tail vitrification. For this, 10 pairs of testis–epididymis complexes were used. The epididymal tails were vitrified using the solid-surface vitrification (SSV) method, in which two vitrification media containing ethylene glycol (EG) 40% or glycerol (GLY) 40% were tested. Vitrification with the presence of EG resulted in better results for all sperm motility parameters (motility, vigour and CASA) compared with GLY (P < 0.05). There were no statistical differences for sperm viability and acrosome integrity, plasma membrane integrity, or overall health of morphologically normal sperm before or after vitrification among experimental groups. In conclusion, epididymal tail vitrification appears to be a suitable method for long-term storage of cat sperm, especially if the procedure is performed with EG as the cryoprotectant.

Keywords

CASAEpididymisFelineSpermatozoaVitrification
Type
Research Article
Information
Zygote , Volume 29 , Issue 6 , December 2021 , pp. 452 - 458
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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References

Ali, J and Shelton, N (2007). Development of vitrification solutions. In: Tucker, MJ and Liebermann, J (eds). Vitrification in Assisted Reproduction – A User’s Manual and Trouble-shooting Guide. Informa Healthcare, London, UK, pp. 45–64.CrossRefGoogle Scholar
Bogliolo, L, Leoni, GG, Ledda, S, Naitana, S, Zedda, M, Carluccio, A and Pau, S (2001). Intracytoplasmic sperm injection of in vitro matured oocytes of domestic cats with frozen-thawed epididymal spermatozoa. Theriogenology 56, 955–67.CrossRefGoogle ScholarPubMed
Brito, DCC, Domingues, SFS, Rodrigues, APR, Maside, C, Lunardi, FO, Wu, X, Figueiredo, JR, Pieczarka, JC and Santos, RR (2018). Cryopreservation of domestic cat (Felis catus) ovarian tissue: comparison of two vitrification methods. Theriogenology 111, 69–77.CrossRefGoogle ScholarPubMed
Buranaamnuay, K (2015). Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa. Reprod Domestic Anim 50, 378–85.CrossRefGoogle ScholarPubMed
Celeghini, ECC, Arruda, RP, Andrade, AFC, Nascimento, J and Raphael, CF (2007). Practical techniques for bovine sperm simultaneous fluorimetric assessment of plasma, acrosomal and mitochondrial membranes. Reprod Domest Anim 42, 479–88.CrossRefGoogle ScholarPubMed
Chatdarong, K, Thuwanut, P, Suksamai, P, Patanatiradaj, S and Sangwornrachasup, A (2009). Survival of frozen-thawed cat spermatozoa pre-cooled in the epididymides. Reprod Domest Anim 44, 377–80.CrossRefGoogle ScholarPubMed
Didion, BA, Dobrinsky, JR, Giles, JR and Graves, CN (1989). Staining procedure to detect viability and the true acrosome reaction in spermatozoa of various species. Gamete Res 22, 51–7.CrossRefGoogle ScholarPubMed
Fahy, GM and Rall, WF (2007). Vitrification: an overview. In: Vitrification in Assisted Reproduction: A User’s Manual and Troubleshooting Guide. Tucker, MJ and Liebermann, J (eds). London, UK, Informa Healthcare, pp. 1–20.Google Scholar
Ganan, N, Gomendio, M and Roldan, ER (2009). Effect of storage of domestic cat (Felis catus) epididymides at 5°C on sperm quality and cryopreservation. Theriogenology 72, 1268–77.CrossRefGoogle Scholar
Hay, MA and Goodrowe, KL (1993). Comparative cryopreservation and capacitation of spermatozoa from epididymides and vasa deferentia of the domestic cat. J Reprod Fertil 47, 297–305.Google ScholarPubMed
Horta, F, Alzobi, H, Jitanantawittaya, S, Catt, S, Chen, P, Pangestu, M and Temple-Smith, P (2017). Minimal volume vitrification of epididymal spermatozoa results in successful in vitro fertilization and embryo development in mice. Asian J Androl 19, 107–12.Google ScholarPubMed
Isachenko, E, Isachenko, V, Katkov, II, Dessole, S and Nawroth, F (2003). Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success. Reprod BioMed 6, 191–200.CrossRefGoogle ScholarPubMed
Jiménez, E, Pérez-Marín, CC, Vizuete, G, Millán, Y and Aguera, EI (2013). Effect of different extenders on in vitro characteristics of feline epididymal sperm during cryopreservation. Reprod Domest Anim 48, 665–72.CrossRefGoogle ScholarPubMed
Karthikeyan, M, Arakkal, D, Mangalaraj, AM and Kamath, MS (2019). Comparison of conventional slow freeze versus permeable cryoprotectant-free vitrification of abnormal semen sample: a randomized controlled trial. J Hum Reprod Sci 12, 150–5.CrossRefGoogle ScholarPubMed
Kashiwazaki, N, Yamaguchi, R, Uesugi, R, Hishiyama, N, Kim, M, Nakatsukasa, E, Kojima, Y, Okuda, Y, Hisamatsu, S, Inomata, T and Shino, M (2005). Sperm motility, plasma membrane integrity, and binding capacity to homologous zona pellucida of cryopreserved epididymal spermatozoa in the domestic cat. J Reprod Dev 51, 735–39.CrossRefGoogle ScholarPubMed
Kedam, T, Katepogu, K and Beeram, E (2016). Effect of cryopreservation on rat epididymis – a histological analysis. J Biomed Sci 5, 2–11.CrossRefGoogle Scholar
Kunkitti, P, Chatdarong, K, Suwimonteerabutr, J, Nedumpun, T, Johannisson, A, Bergqvist, AS, Sjunnesson, Y and Axnér, E (2017). Osmotic tolerance of feline epididymal spermatozoa. Anim Reprod Sci 185, 148–53.CrossRefGoogle ScholarPubMed
Lima, DBC, Silva, TFP, Aquino-Cortez, A, Leiva-Revilla, J and Silva, LDM (2018). Vitrification of testicular tissue from prepubertal cats in cryotubes using different cryoprotectant associations. Theriogenology 110, 110–5.CrossRefGoogle ScholarPubMed
Lima, DBC, Silva, TFP, Aquino-Cortez, A, Pinto, JN, Magalhães, FF, Caldini, BN and Silva, LDM (2016). Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders. Arq Bras Med Vet Zootec 68, 873–81.CrossRefGoogle Scholar
Luvoni, GC (2006). Gamete cryopreservation in the domestic cat. Theriogenology 66, 101–11.CrossRefGoogle ScholarPubMed
Macente, BI, Gutierrez, RR, Apparício, M, Balieiro, CC, Mansano, CFM, Pereira, MM, Borges-Silva, JC, Pires-Buttler, EA, Galvão, ALB, Toniollo, GH, Luvoni, GC, Morselli, MG and Vicente, WRP (2018). Cat epididymal semen cryopreserved with and without vitamin E: effect on sperm parameters and lipid peroxidation. Anim Reprod 15, 1193–8.CrossRefGoogle ScholarPubMed
Martin-Hidalgo, D, Bragado, MJ, Batista, AR, Oliveira, PF and Alves, MG (2019). Antioxidants and male fertility: from molecular studies to clinical evidence. Antioxidants 8, 89.CrossRefGoogle ScholarPubMed
Martins, JL, Villaverde, AI, Lima, AF, Steagall, PV, Ferreira, JC, Taconeli, CA and Lopes, MD (2009). Impact of 24-h cooling prior to freezing on the survival of domestic cat (Felis catus) epididymal sperm. Cat epididymal semen cryopreserved with and without vitamin E: effect on sperm parameters and lipid peroxidation. Reprod Domest Anim 44, 366–8.CrossRefGoogle Scholar
Pradieé, J, Sánchez-Calabuig, MJ, Castaño, C, O’Brien, E, Esteso, MC, Beltran-Brenã, P, Maillo, V, Santiago-Moreno, J and Rizos, D (2018). Fertilizing capacity of vitrified epididymal sperm from Iberian ibex (Capra pyrenaica). Theriogenology 108, 314–20.CrossRefGoogle Scholar
Prochowska, S, Niżański, W, Ochota, M and Partyka, A (2015). Characteristics of urethral and epididymal semen collected from domestic cats – a retrospective study of 214 cases. Theriogenology 84, 1565–71.CrossRefGoogle ScholarPubMed
Prochowska, S, Niżański, W and Partyka, A (2016). Comparative analysis of in vitro characteristics of fresh and frozen–thawed urethral and epididymal spermatozoa from cats (Felis domesticus). Theriogenology 86, 2063–72.CrossRefGoogle Scholar
Ringleb, J, Waurich, R, Wibbelt, G, Streich, WJ and Jewgenow, K (2011). Prolonged storage of epididymal spermatozoa does not affect their capacity to fertilise in vitro-matured domestic cat (Felis catus) oocytes when using ICSI. J Reprod Fertil Dev 23, 818–25.CrossRefGoogle Scholar
Rowlison, T, Ottinger, ME and Comizzoli, P (2018). Key factors enhancing sperm fertilizing ability are transferred from the epididymis to the spermatozoa via epididymosomes in the domestic cat model. J Assist Reprod Genet 35, 221–8.CrossRefGoogle ScholarPubMed
Santos, R, Tharasanit, T, Van Haeften, T, Figueiredo, J, Silva, J and Van den Hurk, R (2007). Vitrification of goat preantral follicles enclosed in ovarian tissue by using conventional and solid-surface vitrification methods. Cell Tissue Res 327, 167–76.CrossRefGoogle ScholarPubMed
Santos, RR, Amorim, C, Cecconi, S, Fassbender, M, Imhoff, M, Lornage, J, Paris, M, Schoenfeldt, V and Martinez-Madrid, B (2010). Cryopreservation of ovarian tissue: an emerging technology for female germline preservation of endangered species and breed. Anim Reprod Sci 122, 151–63.CrossRefGoogle Scholar
Siemieniuch, M and Dubiel, A (2007). Preservation of tomcat (Felis catus) semen in variable temperatures. Anim Reprod Sci 99, 135–44.CrossRefGoogle ScholarPubMed
Spis, E, Bushkovskaia, A, Isachenko, E, Todorov, P, Sanchez, R, Skopets, V and Isachenko, V (2019). Conventional freezing vs. cryoprotectant-free vitrification of epididymal (MESA) and testicular (TESE) spermatozoa: three live births. Cryobiology 90, 100–2.CrossRefGoogle ScholarPubMed
Sringnam, S, Kitlyanant, Y, Lewin, LM and Saikhun, K (2011). Semen quality and chromatin condensation in domestic cat sperm during passage through the epididymis. Kasetsart J Nat Sci 45, 46–58.Google Scholar
Sullivan, R and Saez, F (2013). Epididymosomes, prostasomes, and liposomes: their roles in mammalian male reproductive physiology. Reproduction 146, 21–35.CrossRefGoogle ScholarPubMed
Swanson, W, Bateman, H and Vansandt, L (2017). Urethral catheterization and sperm vitrification for simplified semen banking in felids. Reprod Domest Anim 52, 255–60.CrossRefGoogle ScholarPubMed
Swanson, WF (2006). Application of assisted reproduction for population management in felids: the potential and reality for conservation of small cats. Theriogenology 66, 49–58.CrossRefGoogle ScholarPubMed
Toyonaga, M, Sato, Y, Morita, M, Watanabe, M, Oba, H, Mizutani, T, Hori, T and Tsutsui, T (2010). The qualities of cryopreserved epididymal sperm collected from feline epididymides stored at low temperature. J Vet Med Sci 72, 777–80.CrossRefGoogle ScholarPubMed
Tsutsui, T, Wada, M, Anzai, M and Hori, T (2003). Artificial insemination with frozen epididymal sperm in cats. J Vet Med Sci 65, 397–9.CrossRefGoogle ScholarPubMed
Villaverde, ÀISB, Mello-Martins, MI, Basto-Castro, V and Lopes, MD (2006). Morphological and functional characteristics of chilled semen obtained from domestic feline epididymides (Felis catus). Theriogenology 66, 1641–4.CrossRefGoogle Scholar
Vizuete, G, Jiménez, E, Aguera, EI and Pérez-Marín, CC (2014). Impact of ultra-rapid freezing on the motility, morphology, viability and acrosome integrity of epididymal cat sperm diluted in sucrose-based extenders. Reprod Domest Anim 49, 5–8.CrossRefGoogle ScholarPubMed
Zhou, W, De Iuliis, GN, Dun, MD and Nixon, B (2018). Characteristics of the epididymal luminal environment responsible for sperm maturation and storage. Front Endocrinol 9, 59.CrossRefGoogle ScholarPubMed