Purpose

The aim of the present study was to explore the synergistic effect of nitric oxide, chitosan and gelatin-grafted cotton for wound healing.

Methods

Grafted cottons were prepared by etherification process where hydroxyl group of chitosan and hydroxyl group of cotton formed ether bond with dimethylol dihydroxy ethylene urea.

Results

Photomicrography and SEM studies showed the changes in cotton fibres before and after grafting process. Chitosan-grafted cotton (formulation F1) and chitosan-gelatin-grafted cotton (formulation F2) showed thickening and constriction in fibres after grafting process. Hence, the change in cotton fibres as compared to initial cotton indicated the completion of grafting process. Nitrite contents for formulations F1 and F2 were found to be 90.29% and 92.12%, respectively, with prolonged release of nitric oxide for 24 h. The bonding was confirmed by FTIR study due to strong intra- and inter-hydrogen bonding and weak van der Waals interactions. The bonding of grafted solution to cotton fibre might be due to interaction between various constituent groups of polymer chain with chitosan and cotton. Antimicrobial study of the formulations (F1 and F2) on E. coli and S. aureus revealed that both the formulations showed inhibition on E. coli. Furthermore, results of animal study proved that formulation F2 is synergistically effective when compared to control group as nitric oxide directly affected the wound healing process. In animal study, the formulation F2 showed 70% wound closure in comparison to 55% in formulation F1.

Conclusions

Grafted cotton using etherification process shows potential to become commercial wound healing system in near future.

Graphical abstract

中文翻译：

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一氧化氮对棉花醚化伤口愈合的协同作用

目的

本研究的目的是探讨一氧化氮，壳聚糖和明胶接枝棉对伤口愈合的协同作用。

方法

通过醚化工艺制备接枝棉，其中壳聚糖的羟基和棉的羟基与二羟甲基二羟基乙烯脲形成醚键。

结果

显微照片和SEM研究表明，接枝前后棉纤维的变化。接枝后的壳聚糖接枝棉（配方F1）和壳聚糖-明胶接枝棉（配方F2）显示出纤维的增稠和收缩。因此，与初始棉相比，棉纤维的变化表明接枝过程已完成。发现配方F1和F2的亚硝酸盐含量分别为90.29％和92.12％，并且一氧化氮的释放时间延长了24小时。FTIR研究证实了这种键合，原因是氢键之间和氢键之间的结合力强，范德华相互作用弱。接枝溶液与棉纤维的键合可能是由于聚合物链的各种构成基团与壳聚糖和棉之间的相互作用所致。制剂（F1和F2）的抗微生物研究大肠杆菌和金黄色葡萄球菌显示这两种制剂均显示出对大肠杆菌的抑制作用。此外，动物研究的结果证明，与对照组相比，制剂F2具有协同有效的作用，因为一氧化氮直接影响伤口的愈合过程。在动物研究中，制剂F2的伤口闭合率为70％，而制剂F1为55％。

结论

使用醚化工艺嫁接的棉花显示出在不久的将来成为商业伤口愈合系统的潜力。

图形概要