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Synergistic Effect of Nitric Oxide for Wound Healing Using Etherification of Cotton

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Abstract

Purpose

The aim of the present study was to explore the synergistic effect of nitric oxide, chitosan and gelatin-grafted cotton for wound healing.

Methods

Grafted cottons were prepared by etherification process where hydroxyl group of chitosan and hydroxyl group of cotton formed ether bond with dimethylol dihydroxy ethylene urea.

Results

Photomicrography and SEM studies showed the changes in cotton fibres before and after grafting process. Chitosan-grafted cotton (formulation F1) and chitosan-gelatin-grafted cotton (formulation F2) showed thickening and constriction in fibres after grafting process. Hence, the change in cotton fibres as compared to initial cotton indicated the completion of grafting process. Nitrite contents for formulations F1 and F2 were found to be 90.29% and 92.12%, respectively, with prolonged release of nitric oxide for 24 h. The bonding was confirmed by FTIR study due to strong intra- and inter-hydrogen bonding and weak van der Waals interactions. The bonding of grafted solution to cotton fibre might be due to interaction between various constituent groups of polymer chain with chitosan and cotton. Antimicrobial study of the formulations (F1 and F2) on E. coli and S. aureus revealed that both the formulations showed inhibition on E. coli. Furthermore, results of animal study proved that formulation F2 is synergistically effective when compared to control group as nitric oxide directly affected the wound healing process. In animal study, the formulation F2 showed 70% wound closure in comparison to 55% in formulation F1.

Conclusions

Grafted cotton using etherification process shows potential to become commercial wound healing system in near future.

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References

  1. Witte MB, Barbul A. Role of nitric oxide in wound repair. Am J Surj. 2020;83(4):406–12.

    Google Scholar 

  2. Bindu HT, Vidyavathi M, Kavitha K, Sastry TP, Kumar SRV. Preparation and evaluation of chitosan-gelatin composite films for wound healing activity. Int J Drug Deliv. 2010;2(2):173–82.

    Article  Google Scholar 

  3. Lu B, Wang T, Li Z, Dai F, Lv L, Tang F, Yu K, Liu J, Lan G. Healing of skin wounds with a chitosan–gelatin sponge loaded with tannins and platelet-rich plasma. Int J Biol Macromol. 2016;82:884–91.

    Article  CAS  Google Scholar 

  4. Shende P, Desai A. Impact and scope of intelligent textiles in health care. J Bioequivalence Bioavailab. 2017;9(6):577–84.

    CAS  Google Scholar 

  5. Ambrosio L, Demitri C, Sannino A. Superabsorbent cellulose-based hydrogels for biomedical applications. Biomed Hydrogels. 2011:25–50.

  6. Khalaji MS, Lugoloobi I. Biomedical application of cotton and its derivatives. Cotton Science and Processing Technology. Springer, Singapore; 2020. p. 393–416.

  7. Han G, Nguyen LN, Macherla C, Chi Y, Friedman JM, Nosanchuk JD, Martinez LR. Nitric oxide-releasing nanoparticles accelerate wound healing by promoting fibroblast migration and collagen deposition. Am J Pathol. 2012;180(4):1465–73.

    Article  CAS  Google Scholar 

  8. Kandhwal M, Behl T, Kumar A, Arora S. understanding the potential role and delivery approaches of nitric oxide in chronic wound healing management. Curr Pharm Des. 2020.

  9. Friedman A, Han G, Navati M, Chacko M, Gunther L, Alfieri A, Friedman J. Sustained release nitric oxide releasing nanoparticles: characterization of a novel delivery platform based on nitrite containing hydrogel/glass composites. Nitric Oxide. 2008;19(1):12–20.

    Article  CAS  Google Scholar 

  10. Rizk M, Witte MB, Barbul A. Nitric oxide and wound healing. World J Surg. 2004;28(3):301–6.

    Article  Google Scholar 

  11. Shende PK, Desai D, Gaud RS. Role of solid-gas interface of nanobubbles for therapeutic applications. Crit Rev Ther Drug Carrier Syst. 2018;35(5):469–94.

    Article  Google Scholar 

  12. Singh S, Gupta A, Sharma D, Gupta B. Dextran based herbal nanobiocomposite membranes for scar free wound healing. Int J Biol Macromol. 2018;1(113):227–39.

    Article  Google Scholar 

  13. Hanson SE, Bentz ML, Hematti P. Mesenchymal stem cell therapy for nonhealing cutaneous wounds. Plast Reconstr Surg. 2010;125(2):510.

    Article  CAS  Google Scholar 

  14. Pang C, Fan KS, Wei L, Kolar MK. Gene therapy in wound healing using nanotechnology. Wound Repair Regen. 2020.

  15. Cao J, Wang P, Liu Y, Zhu C, Fan D. Double crosslinked HLC-CCS hydrogel tissue engineering scaffold for skin wound healing. Int J Biol Macromol. 2020;15(155):625–35.

    Article  Google Scholar 

  16. Mittermayr R, Antonic V, Hartinger J, Kaufmann H, Redl H, Téot L, Stojadinovic A, Schaden W. Extracorporeal shock wave therapy (ESWT) for wound healing: technology, mechanisms, and clinical efficacy. Wound Repair Regen. 2012;20(4):456–65.

    PubMed  Google Scholar 

  17. Enoch S, Grey JE, Harding KG. Recent advances and emerging treatments. BMJ. 2006;332(7547):962–5.

    Article  Google Scholar 

  18. Desai D, Shende P. Drug-free Cyclodextrin-based nanosponges for antimicrobial activity. J Pharm Innov. 2020:1–11.

  19. Shende P, Gaud RS. Formulation and comparative characterization of chitosan, gelatin, and chitosan–gelatin-coated liposomes of CPT-11–HCl. Drug Dev Ind Pharm. 2009;35:612–8.

    Article  CAS  Google Scholar 

  20. Soneja A, Drews M, Malinski T. Role of nitric oxide, nitroxidative and oxidative stress in wound healing. Pharmacol Rep. 2005;57:108.

    PubMed  Google Scholar 

  21. Murtey MD, Ramasamy P. Modern electron microscopy in physical and life sciences. Sample Preparations for Scanning Electron Microscopy - Life Sciences. 2016:161–185.

  22. Chung C, Lee M, Choe EK. Characterization of cotton fabric scouring by FT-IR ATR spectroscopy. Carbohydr Polym. 2004;58(4):417–20.

    Article  CAS  Google Scholar 

  23. Hord NG, Ghannam JS, Garg HK, Berens PD, Bryan NS. Nitrate and nitrite content of human, formula, bovine, and soy milks: implications for dietary nitrite and nitrate recommendations. Breastfeed Med. 2001;6(6):393–9.

    Article  Google Scholar 

  24. Masson-Meyers DS, Andrade TA, Caetano GF, Guimaraes FR, Leite MN, Leite SN, Frade MA. Experimental models and methods for cutaneous wound healing assessment Int J. Clin Exp Pathol. 2020;101(1–2):21–37.

    Google Scholar 

  25. Shende P, Gupta H. Formulation and comparative characterization of nanoparticles of curcumin using natural, synthetic and semi-synthetic polymers for wound healing. Life Sci. 2020;253:117588.

    Article  CAS  Google Scholar 

Download references

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Correspondence to Pravin Shende.

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Shende, P., Sahu, P. Synergistic Effect of Nitric Oxide for Wound Healing Using Etherification of Cotton. J Pharm Innov 17, 747–753 (2022). https://doi.org/10.1007/s12247-021-09555-4

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