Abstract

Major studies of the Black Sea algal viruses in Russia have been conducted at scientific institutes located in the city of Sevastopol. A review of the methodological approaches used during 2002–2019 shows that both classical virological methods and methods that were recently developed and patented (among them those by the authors) are applicable to the search for, isolation, and study of algal viruses. The study of samples of clinical material and material of aquatic organisms, as well as model experiments, including those using a unique laboratory apparatus and a test stand, revealed new data on the biology and ecology of marine viruses. Based on the results from these studies, it was proposed to use algal viruses monitoring as a component in aquatic environment monitoring, in which algal viruses are considered as biological indicators and/or markers.

中文翻译：

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黑海藻病毒研究的材料，方法和实验

摘要

俄罗斯的黑海藻类病毒已在塞瓦斯托波尔市的科研机构中进行了重大研究。对2002-2019年期间使用的方法学方法的回顾表明，经典病毒学方法和最近开发并获得专利的方法（其中包括作者的方法）均适用于藻类病毒的搜索，分离和研究。对临床材料和水生生物材料样品的研究以及模型实验（包括使用独特的实验室仪器和测试台的实验）揭示了有关海洋病毒生物学和生态学的新数据。根据这些研究的结果，建议将藻类病毒监测用作水生环境监测的组成部分，