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Isolation, Identification and Characterisation of Fungal Endophytes from Plectranthus amboinicus L.
National Academy Science Letters ( IF 1.2 ) Pub Date : 2021-04-02 , DOI: 10.1007/s40009-020-01035-6
Kanika Chowdhary , Satyawati Sharma , Rishu Kalra

Fungal endophytes of Plectranthus amboinicus L. were explored for their antifungal activity against phytopathogen Sclerotinia sclerotiorum and plant-growth-enhancing traits. Fungal isolates were genotyped using the ITS-rDNA regions. Chaetomium subglobosum exhibited the highest mycelial growth inhibition of 80% against phytopathogen Sclerotinia sclerotiorum in dual-culture bioassay carried out in czapek dox medium. Of the various plant-growth-enhancing traits evaluated in endophytic isolates, C. subglobosum was found to be positive for HCN, IAA, siderophore and ammonia production. None of the endophytic isolates were able to solubilise phosphate in in vitro conditions. IAA was produced by C. subglobosum, Alternaria alternata and Fusarium oxysporum as characterised qualitatively by RP-HPLC. Highest quantity of IAA (97 ng/ml) was secreted by Fusarium oxysporum as detected through spectrophotometric analysis at 530 nm.



中文翻译:

侧柏(Plectranthus amboinicus L.)真菌内生菌的分离,鉴定和表征。

研究了Plectranthus amboinicus L.的真菌内生菌对植物病原菌核盘菌菌核菌的抗真菌活性和增强植物生长的性状。使用ITS-rDNA区域对真菌分离株进行基因分型。毛壳subglobosum显示出80%的最高菌丝体生长的抑制对植物病原体菌核病在双培养生物分析中的Czapek Dox培养基中进行。在内生分离物中评估的各种提高植物生长的性状中,发现球形隐孢子虫对HCN,IAA,铁载体和氨的产生呈阳性。没有一种内生分离物能够在体外溶解磷酸盐条件。通过RP-HPLC定性表征了IAA是由球梭菌链格孢霉镰刀菌产生的。通过在530 nm的分光光度法分析发现,尖孢镰刀菌分泌的IAA量最高(97 ng / ml)。

更新日期:2021-04-04
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