Abstract
Fungal endophytes of Plectranthus amboinicus L. were explored for their antifungal activity against phytopathogen Sclerotinia sclerotiorum and plant-growth-enhancing traits. Fungal isolates were genotyped using the ITS-rDNA regions. Chaetomium subglobosum exhibited the highest mycelial growth inhibition of 80% against phytopathogen Sclerotinia sclerotiorum in dual-culture bioassay carried out in czapek dox medium. Of the various plant-growth-enhancing traits evaluated in endophytic isolates, C. subglobosum was found to be positive for HCN, IAA, siderophore and ammonia production. None of the endophytic isolates were able to solubilise phosphate in in vitro conditions. IAA was produced by C. subglobosum, Alternaria alternata and Fusarium oxysporum as characterised qualitatively by RP-HPLC. Highest quantity of IAA (97 ng/ml) was secreted by Fusarium oxysporum as detected through spectrophotometric analysis at 530 nm.
References
Chowdhary K and Sharma S (2017) Potential of Fungal Endophytes in Plant Growth and Disease Management. In Plant-Microbe Interactions in Agro-Ecological Perspectives (pp. 275-290). Springer, Singapore
Arumugam G, Kumara Swamy M, Sinniah UR (2016) Plectranthusamboinicus (Lour.) Spreng botanical phytochemical pharmacological and nutritional significance. Molecules 21(4):396
Shahab S, Ahmed N, Khan NS (2009) Indole acetic acid production and enhanced plant growth promotion by indigenous PSBs. Afr J Agri Res 4(11):1312–1316
Astuti P, Sudarsono S, Nisak K, Nugroho GW (2014) Endophytic fungi isolated from Coleus amboinicus lour exhibited antimicrobial activity. Adv Pharm Bull 4(Suppl 2):599
Chowdhary K, Kaushik N (2018) Biodiversity study and potential of fungal endophytes of peppermint and effect of their extract on chickpea rot pathogens. Arch Phytopathol Plant Prot 51(3–4):139–155
Chowdhary K, Kaushik N (2019) Biodiversity and In Vitro inhibition study of fungal endophytes of Chlorophytum borivilianum against selected Phytopathogens. Proc Nat AcadSci, India Sect B: BiolSci 89(1):113–121
Kusari P, Kusari S, Spiteller M, Kayser O (2014) Biocontrol potential of endophytesharbored in Radula marginata (liverwort) from the New Zealand ecosystem. Antonie Van Leeuwenhoek. 106(4):771–788
Chowdhary K, Kaushik N (2019) UPLC–MS and dereplication-based identification of metabolites in antifungal extracts of fungal endophytes. Proc Nat AcadSci, India Sect B: BiolSci 89(4):1379–1387
Ruppert KG, Matthew C, McKenzie CM, Popay AJ (2017) Impact of Epichloëendophytes on adult Argentine stem weevil damage to perennial ryegrass seedlings. EntomologiaExperimentalis et Applicata. 163(3):328–337
Gururani MA, Upadhyaya CP, Baskar V, Venkatesh J, Nookaraju A, Park SW (2013) Plant growth-promoting rhizobacteria enhance abiotic stress tolerance in Solanum tuberosum through inducing changes in the expression of ROS-scavenging enzymes and improved photosynthetic performance. J Plant Growth Regul 32(2):245–258
Nath R, Sharma GD, Barooah M (2015) Plant growth promoting endophytic fungi isolated from tea (Camellia sinensis) shrubs of Assam. India. ApplEcol Environ Res. 13:877–891
Khan AR, Ullah I, Waqas M, Shahzad R, Hong SJ, Park GS, Shin JH (2015) Plant growth-promoting potential of endophytic fungi isolated from Solanum nigrum leaves. World J MicrobiolBiotechnol 31(9):1461–1466
Potshangbam M, Devi SI, Sahoo D, Strobel GA (2017) Functional characterization of endophytic fungal community associated with Oryza sativa L and Zea mays L. Front Microbiol 8:325
Ripa FA, Cao WD, Tong S, Sun JG (2019) Assessment of plant growth promoting and abiotic stress tolerance properties of wheat endophytic fungi. BioMed Res Int. https://doi.org/10.1155/2019/6105865
Fatima N, Muhammad SA, Khan I, Qazi MA, Shahzadi I, Mumtaz A, Ismail T (2016) Chaetomium endophytes: a repository of pharmacologically active metabolites. ActaPhysiologiaePlantarum 38(6):136
Acknowledgement
This work was financially supported by the Department of Science and Technology, National Post-doctoral fellowship no. PDF/2016/000317 given to KC. The authors clarify the non-existence of any potential conflict of interest related to the present research.
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Chowdhary, K., Sharma, S. & Kalra, R. Isolation, Identification and Characterisation of Fungal Endophytes from Plectranthus amboinicus L.. Natl. Acad. Sci. Lett. 44, 519–523 (2021). https://doi.org/10.1007/s40009-020-01035-6
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DOI: https://doi.org/10.1007/s40009-020-01035-6