Asparagus (Asparagus officinalis L) is an economically important crop, rich in nutrients, and is also conducive to solving ecological and environmental problems. Plants may acquire benefits from root-associated endophytic bacteria. However, the composition of the endophytic bacterial community associated with the roots of asparagus is poorly elucidated. In this study, the nine root samples of asparagus from three different varieties including Asparagus officinalis var. Grande (GLD), A. officinalis var. Jinglvlu3 (JL3) and A. officinalis var. Jingzilu2 (JZL) were investigated by high-throughput sequencing technology of the 16S rDNA V5-V7 hypervariable region of endophytic bacteria. A total of 16 phyla, 29 classes, 90 orders, 171 families, and 312 genera were identified. Endophytic bacteria diversity and bacteria structure was different among the three varieties and was influenced by rhizosphere soil properties and varieties. In the GLD variety, the main phyla were Proteobacteria, Actinobacteria, and Firmicutes. The main phylum in JL3 and JZL varieties was Proteobacteria. The observations showed that GLD had the highest diversity of endophytes as indicated by the Shannon index (GLD > JZL > JL3). The order of the endophytes richness was GLD > JL3 > JZL. The PCA and PCoA analysis revealed the microbial communities were different between three different asparagus varieties, and the microbial composition of GLD and JZL was more similar. This report provides an important reference for the study of endophytic microorganisms of asparagus.

芦笋（Asparagus officinalis L）是重要的经济作物，营养丰富，也有利于解决生态环境问题。植物可以从与根相关的内生细菌中获益。然而，与芦笋根相关的内生细菌群落的组成尚不清楚。在这项研究中，来自包括Asparagus officinalis var.在内的三个不同品种的九个芦笋根样品。格兰德（GLD），A. officinalis var。Jinglvlu3 (JL3) 和A. officinalis变种。采用高通量测序技术对内生细菌16S rDNA V5-V7高变区进行了研究。共鉴定出16门、29纲、90目、171科、312属。三个品种的内生细菌多样性和细菌结构存在差异，并受根际土壤性质和品种的影响。在GLD品种中，主要的门是变形菌门、放线菌门和厚壁菌门。JL3和JZL品种的主要门是变形菌门. 观察结果表明，如香农指数（GLD > JZL > JL3）所示，GLD 具有最高的内生菌多样性。内生菌丰富度的顺序为GLD > JL3 > JZL。PCA和PCoA分析显示，3个不同芦笋品种的微生物群落不同，GLD和JZL的微生物组成更为相似。该报告为芦笋内生微生物的研究提供了重要参考。