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Root-Associated Endophytic Bacterial Community Composition of Asparagus officinalis of Three Different Varieties

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Abstract

Asparagus (Asparagus officinalis L) is an economically important crop, rich in nutrients, and is also conducive to solving ecological and environmental problems. Plants may acquire benefits from root-associated endophytic bacteria. However, the composition of the endophytic bacterial community associated with the roots of asparagus is poorly elucidated. In this study, the nine root samples of asparagus from three different varieties including Asparagus officinalis var. Grande (GLD), A. officinalis var. Jinglvlu3 (JL3) and A. officinalis var. Jingzilu2 (JZL) were investigated by high-throughput sequencing technology of the 16S rDNA V5-V7 hypervariable region of endophytic bacteria. A total of 16 phyla, 29 classes, 90 orders, 171 families, and 312 genera were identified. Endophytic bacteria diversity and bacteria structure was different among the three varieties and was influenced by rhizosphere soil properties and varieties. In the GLD variety, the main phyla were Proteobacteria, Actinobacteria, and Firmicutes. The main phylum in JL3 and JZL varieties was Proteobacteria. The observations showed that GLD had the highest diversity of endophytes as indicated by the Shannon index (GLD > JZL > JL3). The order of the endophytes richness was GLD > JL3 > JZL. The PCA and PCoA analysis revealed the microbial communities were different between three different asparagus varieties, and the microbial composition of GLD and JZL was more similar. This report provides an important reference for the study of endophytic microorganisms of asparagus.

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Acknowledgements

The present work was supported by National Key Research & Development (R&D) plan (2017YFD0200603), the Construction and Comprehensive Utilization of Germplasm Bank of Biocontrol Germplasm Resources (KJCX20200203), National Natural Science Foundation of China (NSFC 31460031). We thank Majorbio Cloud Platform for their excellent technical assistance.

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Authors and Affiliations

  1. School of Food, Shihezi University, Shihezi, 832000, Xinjiang, China

    Zhuowen Su, Ya Liu & Dianpeng Zhang

  2. Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China

    Zhuowen Su, Shulin Cai, Jianbin Liu, Juan Zhao, Junyu Yin & Dianpeng Zhang

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Su, Z., Cai, S., Liu, J. et al. Root-Associated Endophytic Bacterial Community Composition of Asparagus officinalis of Three Different Varieties. Indian J Microbiol 61, 160–169 (2021). https://doi.org/10.1007/s12088-021-00926-6

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