Banana fruit scarring beetle (BFSB), Basilepta subcostata (Jac.) (Chrysomelidae:Coleoptera), is an important insect pest feeds on leaf and fingers, which affects the cosmetic value of the fruit. The pest is distributed in Assam, Bihar, West Bengal, Chhattisgarh, and North-eastern Hill regions of India. The pest is currently managed by foliar spray with insecticides. In order to identify eco-friendly control of the pest, attempts were made to isolate microbial agent and evaluate their potential to control the pest. A total of 27 entomopathogenic fugal isolates were obtained from Odoiporus longicollis (Oliver), Cosmopolites sordidus (Germar), Basilepta subcostata (Jac), and other insect Galleria mellonella (Fabr). Based on colony morphology, the collected fungal isolates were identified as Metarhizium spp. (17) and Beauveria spp. (10). Through ITS sequencing, the fungal isolates were further characterized at species level as B. bassiana (8), B. brongniartii (2), M. anisopliae (8), M. robertsii (6), M. guizhouense (2), and M. pinghaense (1). Their sequences were submitted in GenBank and obtained accession numbers. Among 27 isolates tested against B. subcostata under laboratory conditions, 3 isolates (M. anisopliae NRCBEPF-36, M. pinghaense NRCBEPF-7 and B. brongniartii NRCBEPF-27) recorded 100% beetle mortality, followed by 11 isolates with 95–99% and 13 isolates with 88–93% within 8 days of treatment. This study highlights the two native North East India isolates B. brongniartii NRCBEPF-27 (MT151781) and M. anisopliae NRCBEPF-36 (MT140308) showed the significance to use as potential biocontrol agents against banana fruit scarring beetle B. subcostata. Further experiments under field conditions are required to evaluate their biological control efficacy against the pest.

中文翻译：

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病原性真菌作为一种有前途的生物防治剂，可对抗香蕉结疤的甲虫巴西勒巴氏杆菌（Jac。）（金眼科：鞘翅目）

香蕉结疤甲虫（Basilepta subcostata（Jac。）（Chrysomelidae：Coleoptera））是一种重要的害虫饲料，主要侵害叶和手指，影响果实的化妆品价值。有害生物分布在印度的阿萨姆邦，比哈尔邦，西孟加拉邦，恰蒂斯加尔邦和东北山地区。目前，该害虫是通过叶面喷洒杀虫剂来防治的。为了确定对有害生物的生态友好控制，已尝试分离微生物制剂并评估其控制有害生物的潜力。共有27种昆虫病原性真菌分离物从长吻O（Odoiporus longicollis，Oliver），Cosmopolites sordidus（Germar），Basilepta subcostata（Jac）和其他昆虫盖勒梅勒菌（Galleria mellonella，Fabr）获得。基于菌落形态，收集的真菌分离物被鉴定为Metarhizium spp。（17）和白僵菌属。（10）。通过ITS测序，进一步将真菌分离物在物种水平上表征为球孢白僵菌（B. bassiana）（8），布朗氏芽孢杆菌（B. brongniartii）（2），芒分枝杆菌（M. anisopliae）（8），罗伯茨氏菌（M. robertsii）（6），贵州分枝（M. guizhouense）（ M. pinghaense（1）。它们的序列已提交GenBank并获得了登录号。在实验室条件下测试的27种对肋旁芽孢杆菌的分离株中，3种分离株（M. anisopliae NRCBEPF-36，M. pinghaense NRCBEPF-7和B. brongniartii NRCBEPF-27）的甲虫死亡率均为100％，其次是11种95-99的菌株治疗后8天内，有13％的细菌和88-93％的细菌分离株。这项研究突出了印度东北部的两个本地分离株，如B. brongniartii NRCBEPF-27（MT151781）和M. anisopliae NRCBEPF-36（MT140308），显示出作为潜在的生物防治剂对抗香蕉结疤的甲虫B. subcostata的重要性。