The growth of plant roots is regulated by various factors. Brassinosteroids (BRs) can induce asymmetric root growth (ARG) to form waves and coils, and this phenomenon can be eliminated by ethylene (ETH) inhibitors aminoacetic acid (AOA) or silver thiosulfate (STS), indicating this process depends on ETH pathway. Nevertheless, the research on related genes has not been reported. In this study, four treatments (Water, Brassinolide (BL), BL + AOA, and BL + STS) were set up using the seedlings of Oryza sativa as materials. The differentially expressed genes (DEGs) in BR-induced ARG via ETH pathway were identified by transcriptome sequencing, weighted gene co-expression network analysis (WGCNA), and real-time quantitative PCR (qRT-PCR). Finally, we screened 70 DEGs, including ALPHA-EXPANSINs (OsEXPAs), WALL-ASSOCIATED RECEPTOR KINASE-LIKE 16 (OsWAKL16), and TRANSMEMBRANE KINASE (OsTMK) that regulated cell elongation and expansion, as well as plant hormone synthase genes, 1-AMINO-CYCLOPROPANE-1-CARBOXYLIC ACID OXIDASEs (OsACOs), OsYUCCAs, and CYTOKININ OXIDASE(OsCKX). Most of them were up-regulated by BRs via ETH pathway. In addition, the receptor-like kinase (RLK) gene ROOT MEADNDER CURLING (OsRMC), a negative regulator in jasmonic acid-induced ARG, could be inhibited by BRs, and this process also depended on ETH pathway. The identification of the above genes provides evidence for revealing the molecular mechanism in the process.

植物根的生长受多种因素调节。油菜素类固醇（BRs）可以诱导不对称根生长（ARG）形成波浪和线圈，这种现象可以通过乙烯（ETH）抑制剂氨基乙酸（AOA）或硫代硫酸银（STS）消除，表明该过程取决于ETH途径。尽管如此，有关基因的研究尚未见报道。在这项研究中，以稻的幼苗为材料，建立了四种处理方法（水，油菜素内酯（BL），BL + AOA和BL + STS）。通过转录组测序，加权基因共表达网络分析（WGCNA）和实时定量PCR（qRT-PCR），鉴定了通过ETH途径在BR诱导的ARG中的差异表达基因（DEG）。最后，我们筛选了70个DEG，包括ALPHA-EXPANSIN（OsEXPAs），壁相关受体激酶样16（OsWAKL16）和跨膜激酶（OsTMK）调节细胞的伸长和扩增，以及植物激素合酶基因，1-氨基环丙烷-1羧酸氧化酶（O），OsYUCCA和细胞分裂素氧化酶（OsCKX）。它们中的大多数被BR通过ETH途径上调。此外，受体样激酶（RLK）基因ROOT MEADNDER CURLING（OsRMC），茉莉酸诱导的ARG中的负调节剂，可能被BRs抑制，这一过程也取决于ETH途径。上述基因的鉴定为揭示该过程中的分子机理提供了证据。