Droplet microfluidics is a powerful tool for a diverse range of biomedical and industrial applications such as single-cell biology, synthetic biology, digital PCR, biosafety monitoring, drug screening, and food, feed, and cosmetic industries. As an integral part of droplet microfluidics, on-chip multiplexed droplet sorting has recently gained enthusiasm, since it enables real-time sorting of single droplets containing cells with different phenotypes into multiple bins. However, conventional sorting methods are limited in throughput and scalability. Here, we present high-throughput, scalable, multiplexed droplet sorting by employing a pair of sequentially addressable dielectrophoretic arrays (SADAs) across a microchannel on a microfluidic chip. A SADA is an on-chip array of electrodes, each of which is sequentially activated and deactivated in synchronization to the position and speed of a flowing droplet of interest. The dual-SADA (dSADA) structure enables high-throughput deflection of droplets in multiple directions in a well-controlled manner. For proof-of-concept demonstration and characterization of the dSADA, we performed fluorescence-activated droplet sorting (FADS) with a 3-way dSADA at a high throughput of 2450 droplets/s. Furthermore, to show the scalability of the dSADA, we also performed FADS with a 5-way dSADA at a high throughput of 473 droplets/s.

液滴微流控技术是用于多种生物医学和工业应用的强大工具，例如单细胞生物学，合成生物学，数字PCR，生物安全监测，药物筛选以及食品，饲料和化妆品工业。作为液滴微流控不可或缺的一部分，片上多路复用液滴分选最近获得了极大的热情，因为它能够将包含具有不同表型细胞的单个液滴实时分选到多个容器中。但是，传统的分类方法在吞吐量和可伸缩性方面受到限制。在这里，我们通过在微流控芯片上的微通道上使用一对顺序可寻址介电电泳阵列（SADAs），提出了高通量，可扩展，多路复用的液滴分选。SADA是电极的芯片上阵列，每一个都与感兴趣的小滴的位置和速度同步地顺序激活和停用。双SADA（dSADA）结构能够以良好控制的方式使液滴在多个方向上实现高通量偏转。为了进行dSADA的概念验证演示和表征，我们使用3向dSADA以2450滴/秒的高通量进行了荧光激活的液滴分选（FADS）。此外，为了显示dSADA的可扩展性，我们还使用5路dSADA进行了FADS，并以473个小滴/秒的高通量进行了分析。我们使用3路dSADA进行了荧光激活的液滴分选（FADS），其高通量为2450液滴/秒。此外，为了显示dSADA的可扩展性，我们还使用5路dSADA进行了FADS，并以473个小滴/秒的高通量进行了分析。我们使用3路dSADA进行了荧光激活的液滴分选（FADS），其高通量为2450液滴/秒。此外，为了显示dSADA的可扩展性，我们还使用5路dSADA进行了FADS，并以473个小滴/秒的高通量进行了分析。