The green synthesized nanoparticles have been determined as a novel pesticide against arthropod pests. This study was designed to evaluate the in vitro acaricidal activity of green synthesized nickel oxide nanoparticles (NiO NPs) using aqueous extract of Melia azedarach ripened fruits against different developmental stages of the camel tick Hyalomma dromedarii in addition to their toxic effect on laboratory animals. The synthesized NiO NPs were characterized by UV–visible (UV–Vis) spectroscopy, Fourier transforms infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). The UV–Vis spectra of the NiO NPs showed an absorption peak at 307 nm. FTIR analysis showed the possible functional groups used for capping and stabilization of NiO NPs with strong bands at 3416.2 and 1626.6 cm⁻¹. The SEM images of the NiO NPs exhibited a size ranging from 21 to 35 nm. The immersion test was used for the in vitro application of the synthesized NiO NPs on the various tick stages (egg, nymph, larva, and adult). Mortality percentages and LC₅₀ values of each tick stage were calculated. The oviposition and hatchability of the engorged females were monitored for the survived tick after treatment. The LC₅₀ values for NiO NPs on embryonated eggs, larvae, and engorged nymphs were 5.00, 7.15, and 1.90 mg/mL, respectively. The egg productive index (EPI), egg number, and hatchability (%) were lower in females treated with the NiO NPs than in control ticks. The toxicity of the NiO NPs on laboratory animals was also investigated using Swiss albino mice by oral dose of 500 mg/kg/day administration for five consecutive days. The hematological, biochemical, and histopathological changes were evaluated. The hematological analysis showed significant increase in the level of white blood cells (WBC) and hemoglobin (Hb). Biochemical analysis showed non-significant decrease in alkaline phosphatase (ALP) and alanine amino transferase (ALT). We concluded that NiO NPs have a significant acaricidal activity as demonstrated on eggs, larvae, engorged nymphs, and fully fed females of H. dromedarii. From a toxicological point of view further in vivo investigations are needed to determine the mechanism of toxic effect of NiO NPs.

绿色合成纳米颗粒已被确定为一种新型的节肢动物害虫杀虫剂。这项研究旨在评估使用美丽ia成熟果实的水提取物对骆驼壁tick的不同发育阶段的绿色合成氧化镍纳米颗粒（NiO NPs）的体外杀螨活性。除了对实验动物有毒作用外。合成的NiO NPs的特征在于紫外可见光谱（UV-Vis），傅立叶变换红外光谱（FTIR），扫描电子显微镜（SEM）和能量色散X射线光谱（EDS）。NiO NPs的UV-Vis光谱在307 nm处显示吸收峰。FTIR分析表明，可能的官能团可用于在3416.2和1626.6 cm ^-1处具有强谱带的NiO NPs的封端和稳定化。NiO NP的SEM图像显示尺寸范围为21到35 nm。浸没测试用于将合成的NiO NPs体外应用到不同的壁虱阶段（例如卵，若虫，幼虫和成虫）。死亡率和LC ₅₀计算每个刻度阶段的值。监测充血雌性的产卵和孵化后的存活treatment。LC ₅₀胚卵，幼虫和若虫若虫的NiO NPs值分别为5.00、7.15和1.90 mg / mL。用NiO NPs处理的雌性的产蛋指数（EPI），卵数和孵化率（％）均低于对照tick。还使用瑞士白化病小鼠通过连续五天口服500 mg / kg /天的剂量研究了NiO NP对实验动物的毒性。评估了血液学，生化和组织病理学变化。血液学分析显示白细胞（WBC）和血红蛋白（Hb）的水平显着增加。生化分析表明，碱性磷酸酶（ALP）和丙氨酸氨基转移酶（ALT）的下降不明显。我们得出的结论是，NiO NP在卵，幼虫，若虫，若虫，dromedarii。从毒理学的角度来看，需要进一步的体内研究以确定NiO NPs的毒性作用机理。