Abstract

Electrophoretically homogeneous lectin with a specific activity of 100.0 U/mg protein was isolated and purified from the fungal mycelium of Fusarium solani 4. The molecular weight of the native lectin molecule (38 kDa) was determined. It was established that it consists of two identical subunits, with the molecular mass determined via PAGE-Na electrophoresis in the presence of DDS-Na equal to 19 kDa. The physicochemical properties and carbohydrate specificity of the lectin, as well as its in vitro effect on mammalian cancer cells, were studied. The results opened new prospects for the further study of lectin and its potential applications as a medical agent.

中文翻译：

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真菌Fusarium solani 4中凝集素的分离，纯化和鉴定

摘要

从枯萎镰刀菌（Fusarium solani） 4的真菌菌丝体中分离并纯化了具有100.0 U / mg蛋白比活的电泳均质凝集素。测定了天然凝集素分子的分子量（38 kDa）。已经确定它由两个相同的亚基组成，在DDS-Na存在下，通过PAGE-Na电泳测定的分子量等于19 kDa。研究了凝集素的理化性质和碳水化合物特异性，以及其对哺乳动物癌细胞的体外作用。该结果为进一步研究凝集素及其作为药物的潜在应用开辟了新的前景。