Abstract
Electrophoretically homogeneous lectin with a specific activity of 100.0 U/mg protein was isolated and purified from the fungal mycelium of Fusarium solani 4. The molecular weight of the native lectin molecule (38 kDa) was determined. It was established that it consists of two identical subunits, with the molecular mass determined via PAGE-Na electrophoresis in the presence of DDS-Na equal to 19 kDa. The physicochemical properties and carbohydrate specificity of the lectin, as well as its in vitro effect on mammalian cancer cells, were studied. The results opened new prospects for the further study of lectin and its potential applications as a medical agent.
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ACKNOWLEDGMENTS
The authors are grateful to A.A. Nabatov (Department of Biochemistry and Clinical Laboratory Diagnostics, Kazan State Medical Academy) for help and helpful advice with analytical experiments.
Funding
This work was carried out with financial support from the 8th competition “Fifty Best Innovative Ideas for the Republic of Tatarstan” and the program “Participant of the Youth Scientific and Innovative Competition” (project 11717r/17263).
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Mukhammadiev, R.S., Mukhammadiev, R.S., Skvortsov, E.V. et al. Isolation, Purification, and Characterization of a Lectin from the Fungus Fusarium solani 4. Appl Biochem Microbiol 57, 206–211 (2021). https://doi.org/10.1134/S0003683821020095
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DOI: https://doi.org/10.1134/S0003683821020095