Abstract

Main application of microfluidic paper-based analytical devices (µPADs) is for low-cost and portable assays. In order to reduce the cost, miniaturization seems to be an effective way to save reagents, samples, materials, etc. Present study demonstrates that mini-µPADs based on nitrocellulose membrane with only 2 main channels can perform sandwich enzyme-linked immunosorbent assays (ELISA) for quantitative determination of human chorionic gonadotropin (hCG). Moreover, the width and the length of these devices are only about 4.0 and 33.4 mm, respectively. Inkjet printing technology, having the advantages of simple and material-saving process, was used to create hydrophobic barrier. Colorimetric signals from ELISA captured by digital camera and measured by ImageJ software shows that these µPADs can quantitative determine of hCG in the range from 5 to 10 000 ng/mL. Furthermore, duration for each tests is only about 5 min. The success in fabricating miniaturized µPADs shows the potentials to reduce the size of µPADs as well as the amount of needed samples, time and reagents. For all these reasons, this miniaturization concept can be applied to many further applications of analytical biochemical assays.

中文翻译：

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基于INKJET印刷的用于三明治式酶联免疫吸附测定的基于微流控纸的微型分析仪器的制备

摘要

微流体纸质分析设备（µPAD）的主要应用是低成本和便携式分析。为了降低成本，小型化似乎是节省试剂，样品，材料等的有效方法。本研究表明，仅具有两个主要通道的基于硝化纤维素膜的mini-µPAD可以进行夹心酶联免疫吸附测定（ELISA） ）定量测定人绒毛膜促性腺激素（hCG）。而且，这些装置的宽度和长度分别仅为大约4.0mm和33.4mm。喷墨打印技术具有简单且节省材料的优点，可用于创建疏水性屏障。用数码相机捕获并通过ImageJ软件测量的ELISA比色信号表明，这些µPAD可以定量测定hCG，范围为5至10000 ng / mL。此外，每个测试的持续时间仅约5分钟。微型µPAD的成功制造显示出减小µPAD尺寸以及所需样品，时间和试剂数量的潜力。由于所有这些原因，这种小型化概念可以应用于分析生化分析的许多其他应用。