Abstract—

Formation of a community of phosphate-accumulating microorganisms in a laboratory sequential batch reactor (SBR) ensuring alternated aerobic and anaerobic conditions during periodic removal and addition of the medium were investigated. The bioreactor removed 50% phosphorus from the incoming medium after 22 days from the start-up. Microscopy and X-ray microassay revealed the of cells of diverse morphology that contained phosphorus-enriched granules. High-throughput sequencing of the 16S rRNA gene fragments carried out on days 0, 8, 15, and 22 showed changes in the community composition and its decreasing diversity. On day 22, approximately twofold increase of the relative abundances of Bacteroidetes (up to 43% of the 16S rRNA gene sequences) and Proteobacteria of the classes alpha (up to 15%) and beta (up to 27%) was observed. While at the onset of the reactor operation, typical PAOs related to “Candidatus Accumulibacter” (class Betaproteobacteria) constituted 0.2% of the community, they were not detected on day 22. The most likely PAO candidates were beta-proteobacteria of the genus Dechloromonas, the share of which increased from 0.7 to 11% by the time of the highest phosphorus removal from the inflowing medium. The relative abundance of heterotrophs of the genus Zoogloea (family Rhodocyclaceae) increased from 0.1 to 11.5%.

中文翻译：

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循环有氧-厌氧培养条件下去除磷酸盐的微生物联合体

摘要—

研究了在实验室顺序分批反应器（SBR）中形成的磷酸盐积累型微生物群落的形成，以确保在定期去除和添加培养基期间交替出现好氧和厌氧条件。在启动22天后，生物反应器从进入的培养基中去除了50％的磷。显微镜检查和X射线显微测定揭示了含有富含磷的颗粒的多种形态的细胞。在第0、8、15和22天进行的16S rRNA基因片段的高通量测序显示了群落组成的变化及其多样性的降低。在第22天，大约两倍增加的相对丰度的拟杆菌（最多的16S rRNA基因序列的43％）和变形杆菌纲观察到α（高达15％）和β（高达27％）类别中的一半。而在反应堆运行的开始，与“典型的PAO暂定 Accumulibacter”（类β-变形菌）构成社会的0.2％，他们并没有在22日检测到的最有可能的PAO候选人属的β-变形菌脱氯，从流入的介质中去除磷的最高时间，其份额从0.7％增加到11％。兽足类（Rhodocyclaceae）属的异养菌的相对丰度从0.1增加到11.5％。