Abstract

Lipopolysaccharide (LPS) of a new Pantoea agglomerans strain P324 was studied by chemical and biological methods. Mild acid hydrolysis of the LPS resulted in lipid A and O-specific polysaccharide (OPS) fractions. Studies by negative-ion mode HR ESI mass spectrometry showed heterogeneity of the lipid A, the major form being a hexa-acylated derivative containing biphosphorylated GlcN disaccharide, four 14:0 (3-OH), one 18:0, and one 12:0 residues. The following structure of the OPS was elucidated by chemical, NMR and computational methods: →3)-α-L-Rhap-(1→4)-α-D-Glcp-(1→. The P. agglomerans P324 LPS showed medium level of toxic and pyrogenic activities. Structural components of the LPS exhibited varying effects on the activity of Bacillus peptidases. Thus, the OPS and lipid A played a significant role in the hydrolysis of fibrin by Bacillus proteases but did not affect the activity of protease 2 of B. thuringiensis IMV B-7465 and protease 1 of B. thuringiensis IMV B-7324. Hydrolysis of elastin was intensified by core oligosaccharide and lipid A. Hydrolysis of collagen in the presence of the isolated fractions was accompanied by the inhibition of activity as compared to the native LPS.

中文翻译：

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团聚体P324的O-特异性多糖和脂质a的结构和生物学特性

摘要

通过化学和生物学方法研究了新的Pantoea agglomerans菌株P324的脂多糖（LPS）。LPS的轻度酸水解产生脂质A和O特异性多糖（OPS）馏分。负离子模式HR ESI质谱研究显示脂质A的异质性，主要形式为六酰化衍生物，其中含有双磷酸化GlcN二糖，四个14：0（3-OH），一个18：0和一个12： 0个残基。OPS的下列结构阐明通过化学，NMR和计算方法：→3）-α-L-鼠李糖p - （1→4）-α-d-Glcp-（1→的P.成团P324 LPS显示中等水平的有毒和热原活性LPS的结构成分对LPS的活性表现出不同的影响芽孢杆菌肽酶。因此，OPS和脂质A在芽孢杆菌蛋白酶水解纤维蛋白中起重要作用，但不影响苏云金芽孢杆菌IMV B-7465的蛋白酶2和苏云金芽孢杆菌IMV B-7324的蛋白酶1的活性。弹性寡糖和脂质A增强了弹性蛋白的水解。与天然LPS相比，在分离的组分存在下，胶原蛋白的水解伴随着活性的抑制。