Trichoderma aggressivum, a mycopathogen causing green mould disease, is a major problem in Agaricus bisporus cultivation due to crop loss, and resistance to chemical fungicides. There is an urgent need for novel biological ways to control mycopathogens without affecting the growth of A. bisporus. Bacteria from the mushroom-casing environment were identified and tested for antagonistic effect on T. aggressivum. Bacillus velezensis produced a large zone of inhibition and its supernatant inhibited the growth of T. aggressivum [−37%], and slightly stimulated A. bisporus growth [+2%]. Label free quantitative-proteomic (LFQ) analysis of changes in the abundance of T. aggressivum proteins following exposure to B. velezensis supernatant indicated increased abundance of proteins associated with catabolic processing of amino acids (40-fold), amino oxidase proteins (14-fold), oxidoreductase proteins (13-fold, 4-fold) and hydrolases (3-fold). Proteins that decreased in relative abundance were antioxidants (29-fold), NTF2 domain containing protein (17-fold), 60S ribosomal protein L-13 (14-fold), glucoamylase proteins (13-fold), proteasome subunit proteins (11-fold) and other ribosomal proteins (9-fold). LFQ analysis revealed that exposing A. bisporus to B. velezensis supernatant led to a decrease in: prohibitin (13-fold, 6-fold), proteasomal proteins (11-fold), cytosolic adaptor domain containing protein (5-fold), aldehyde dehydrogenase (4-fold), ribosomal proteins (4-fold), DLH domain-containing protein (4-fold) and PKS_ER domain containing protein (3-fold). The results indicate that A. bisporus was not under stress upon contact with B. velezensis. Whereas a detrimental effect of B. velezensis on T. aggressivum is shown by inhibition of growth and damage-preventing proteins and increased abundance of proteins associated with stress.

引起绿色霉菌病的致病菌木霉agrogressivum是双孢蘑菇栽培中的一个主要问题，原因是农作物损失并且对化学杀真菌剂产生抗性。迫切需要一种新的生物学方法来控制真菌病原菌而不影响双孢曲霉的生长。鉴定了来自蘑菇套管环境的细菌，并测试了其对侵略性铁锈菌的拮抗作用。velezensis芽孢杆菌产生了很大的抑制区，其上清液抑制了侵略性拟南芥的生长[-37％]，并稍微刺激了双孢曲霉的生长[+ 2％]。标记变化的自由定量蛋白质组学（LFQ）分析中的丰度的T. aggressivum暴露于velezensis上清液中的蛋白质表明与氨基酸分解代谢加工相关的蛋白质丰度增加（40倍），氨基氧化酶蛋白质（14倍），氧化还原酶蛋白质（13倍，4倍）和水解酶（3 -折叠）。相对丰度降低的蛋白质是抗氧化剂（29倍），含NTF2结构域的蛋白质（17倍），60S核糖体蛋白L-13（14倍），葡糖淀粉酶蛋白质（13倍），蛋白酶体亚基蛋白质（11-倍）和其他核糖体蛋白（9倍）。LFQ分析表明，双孢蘑菇暴露于velezensis上清液导致以下物质的减少：禁止素（13倍，6倍），蛋白酶体蛋白质（11倍），含有胞质衔接子结构域的蛋白质（5倍），醛脱氢酶（4倍），核糖体蛋白质（4折叠），含DLH结构域的蛋白质（4倍）和含PKS_ER结构域的蛋白质（3倍）。结果表明，双孢霉与velezensis接触时没有受到胁迫。然而，通过抑制生长和防止损伤的蛋白质以及增加与应激有关的蛋白质的丰度，可以显示出velezensis对侵略性T. agressiveum的有害影响。