Abstract

The TREX-2 protein complex is the key participant in the export of mRNA from the nucleus to the cytoplasm through the nuclear pores. Previously, a protein complex of D. melanogaster consisting of TREX-2 and ORC complexes was purified. It was shown that, in the TREX-2-ORC complex, the Xmas-2 protein, which is the platform for TREX-2 assembly, interacts with the Orc3 protein. The aim of this work was to investigate what regions of the Xmas-2 amino acid sequence are involved in the interaction with Orc3. It was shown that the interaction of Xmas-2 with Orc3 requires a C-terminal region of Xmas-2 located downstream of the CID domain.

中文翻译：

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研究TREX-2 mRNA出口复合物的主要蛋白质Xmas-2与黑腹果蝇ORC复合物的一个亚基Orc3蛋白之间的相互作用。

摘要

TREX-2蛋白复合物是mRNA从细胞核通过核孔向细胞质输出的主要参与者。先前，纯化了由TREX-2和ORC复合物组成的黑腹果蝇的蛋白质复合物。结果表明，在TREX-2-ORC复合物中，作为TREX-2组装平台的Xmas-2蛋白与Orc3蛋白相互作用。这项工作的目的是调查Xmas-2氨基酸序列的哪些区域参与与Orc3的相互作用。结果表明，Xmas-2与Orc3的相互作用需要Xmas-2的C端区域位于CID域的下游。