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Study of the Interaction between Xmas-2, the Main Protein of TREX-2 mRNA Export Complex, and the Orc3 Protein, a Subunit of ORC Complex of D. melanogaster

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

The TREX-2 protein complex is the key participant in the export of mRNA from the nucleus to the cytoplasm through the nuclear pores. Previously, a protein complex of D. melanogaster consisting of TREX-2 and ORC complexes was purified. It was shown that, in the TREX-2-ORC complex, the Xmas-2 protein, which is the platform for TREX-2 assembly, interacts with the Orc3 protein. The aim of this work was to investigate what regions of the Xmas-2 amino acid sequence are involved in the interaction with Orc3. It was shown that the interaction of  Xmas-2 with Orc3 requires a C-terminal region of  Xmas-2 located downstream of the CID domain.

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ACKNOWLEDGMENTS

The study was performed using the equipment of the Core Facility of the Institute of Gene Biology, Russian Academy of Sciences.

Funding

This work was supported by the Russian Foundation for Basic Research (project no. 19-04-00861).

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia

    M. M. Kurshakova, D. V. Kopytova &  S. G. Georgieva

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  1. M. M. Kurshakova
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  2. D. V. Kopytova
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  3. S. G. Georgieva
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Correspondence to M. M. Kurshakova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Kurshakova, M.M., Kopytova, D.V. & Georgieva, S.G. Study of the Interaction between Xmas-2, the Main Protein of TREX-2 mRNA Export Complex, and the Orc3 Protein, a Subunit of ORC Complex of D. melanogaster . Dokl Biochem Biophys 496, 18–21 (2021). https://doi.org/10.1134/S1607672921010063

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  • DOI: https://doi.org/10.1134/S1607672921010063

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