Purpose

Trichosporon inkin is recognized as one of the vital causative organisms of white piedra in humans. Very few herbal treatments are available against white piedra. Muntingia calabura (MC) leaf extract and silver (Ag) nanoparticles are identified for their numerous therapeutic applications which is the main purpose of this research work.

Methods

In this work, we synthesized, characterized, and studied the stability of Ag nanoparticles embedded in the aqueous extract of MC leaf. The antifungal and antioxidant activity of the Ag nanoparticles embedded MC leaf extract was examined using well diffusion method and 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, respectively.

Results

Absorbance peak at 440 nm in UV-visible spectrum confirmed the reduction of silver ions to silver nanoparticles. Dynamic light scattering (DLS) zeta potential analyzer revealed the average particle size of the nanoparticles as 20 nm with the zeta potential of − 18.9 mV. Scanning electron microscope (SEM) analysis showed spherical nanoparticles with a particle size of 20 to 50 nm. Elemental analysis using energy-dispersive x-ray analysis (EDX) identified the presence of Ag. Fourier transforms infrared (FTIR) spectroscopy results predicted functional groups of MC leaf extract and Ag nanoparticles functionalized with MC extract explaining the interaction between them. The crystallinity of silver nanoparticles and particle size was identified using an x-ray diffractometer (XRD).

Conclusions

Ag nanoparticles embedded in MC leaf extract exhibited good stability and possessed good antioxidant activity. The zone of inhibition inferred for the Ag nanoparticles embedded in MC leaf extract determined its antifungal activity against Trichosporon inkin. Hence, the developed Ag nanoparticles embedded in MC leaf extract can be used efficiently in any formulations formulated against Trichosporon inkin.

中文翻译：

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芒白叶提取物中银纳米颗粒固化白皮德拉的制备与评价

目的

cho曲霉菌素被认为是人类白色皮德拉的重要致病生物之一。很少有草药治疗白皮德拉。鉴定出Muntingia calabura（MC）叶提取物和银（Ag）纳米颗粒具有众多治疗用途，这是本研究工作的主要目的。

方法

在这项工作中，我们合成，表征和研究了嵌入MC叶水提取物中的Ag纳米颗粒的稳定性。分别采用孔扩散法和2，2二苯基-1-吡啶并肼基（DPPH）自由基清除法检测了包埋有Ag纳米颗粒的MC叶提取物的抗真菌和抗氧化活性。

结果

紫外可见光谱中440 nm处的吸光度峰证实了银离子向银纳米颗粒的还原。动态光散射（DLS）ζ电位分析仪显示纳米粒子的平均粒径为20 nm，ζ电位为− 18.9 mV。扫描电子显微镜（SEM）分析显示了粒径为20至50nm的球形纳米颗粒。使用能量色散X射线分析（EDX）进行的元素分析确定了Ag的存在。傅里叶变换红外（FTIR）光谱结果预测了MC叶提取物的功能基团以及用MC提取物功能化的Ag纳米颗粒，从而解释了它们之间的相互作用。使用X射线衍射仪（XRD）识别银纳米颗粒的结晶度和粒径。

结论

嵌入MC叶提取物中的Ag纳米颗粒表现出良好的稳定性并具有良好的抗氧化活性。推断MC叶提取物中嵌入的Ag纳米颗粒的抑制作用区决定了其对毛孢霉菌的抗真菌活性。因此，嵌入MC叶提取物中的已开发的Ag纳米颗粒可以有效地用于针对付Trichosporon inkin配制的任何制剂。