Resveratrol has a variety of biological functions, however, a limited number of studies have assessed its interaction with cell surface receptors. In this study, a sandwich-type rat small intestine tissue sensor (RSIT-sensor) was fabricated to detect the response current from receptor stimulation by different resveratrol concentrations via electrochemical workstation. The results showed that with detection limit of 1 × 10^–13 mol/L, the maximum rate of change of the response current was found at the concentration of 8.5 × 10^–12 mol/L, indicating that the resveratrol-related receptor was saturated. With comparing the response values of prepared biosensor and bare electrode with resveratrol, it can be concluded that the response value of small intestinal cells to resveratrol has obviously been amplified by the intracellular signal transmission system, and its magnification was about 100 times. In the current research, for the first time, kinetics of the interaction between resveratrol and its receptors and the transmission of signals to the body could be quantitatively measured by a biosensor. Our findings may provide new ideas for resveratrol-related receptor analysis, separation and purification, signal transmission, and evaluation of biological function.

白藜芦醇具有多种生物学功能，然而，有限数量的研究评估了其与细胞表面受体的相互作用。在本研究中，制备了夹心型大鼠小肠组织传感器（RSIT 传感器），通过电化学工作站检测不同浓度白藜芦醇对受体刺激的响应电流。结果表明，在检测限为 1 × 10 ^–13  mol/L 时，响应电流的最大变化率出现在浓度为 8.5 × 10 ^–12 mol/L，表明白藜芦醇相关受体已饱和。通过比较制备的生物传感器和白藜芦醇裸电极的响应值，可以得出结论，小肠细胞对白藜芦醇的响应值被细胞内信号传输系统明显放大，其放大倍数约为100倍。在目前的研究中，白藜芦醇与其受体之间的相互作用以及向身体传输信号的动力学首次可以通过生物传感器进行定量测量。我们的发现可能为白藜芦醇相关受体分析、分离纯化、信号传输和生物功能评估提供新思路。