Abstract
Resveratrol has a variety of biological functions, however, a limited number of studies have assessed its interaction with cell surface receptors. In this study, a sandwich-type rat small intestine tissue sensor (RSIT-sensor) was fabricated to detect the response current from receptor stimulation by different resveratrol concentrations via electrochemical workstation. The results showed that with detection limit of 1 × 10–13 mol/L, the maximum rate of change of the response current was found at the concentration of 8.5 × 10–12 mol/L, indicating that the resveratrol-related receptor was saturated. With comparing the response values of prepared biosensor and bare electrode with resveratrol, it can be concluded that the response value of small intestinal cells to resveratrol has obviously been amplified by the intracellular signal transmission system, and its magnification was about 100 times. In the current research, for the first time, kinetics of the interaction between resveratrol and its receptors and the transmission of signals to the body could be quantitatively measured by a biosensor. Our findings may provide new ideas for resveratrol-related receptor analysis, separation and purification, signal transmission, and evaluation of biological function.
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This study was financially supported by the National Natural Science Foundation of China (Grant No. 31901782).
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Ruijuan Ren participated in drafting the manuscript, as well as revision. Tingting Liu contributed to study design and drafting the primary version of the manuscript. The study was conducted under Dingqiang Lu’s supervision. All authors have read and approved the submitted version of the manuscript.
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Ren, R., Lu, D. & Liu, T. Development of a sandwich-type rat small intestine tissue sensor for detecting resveratrol and its receptors. Biomed Microdevices 23, 13 (2021). https://doi.org/10.1007/s10544-021-00554-3
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DOI: https://doi.org/10.1007/s10544-021-00554-3