Five accessions representing divergent lineages of wild peas (Pisum sativum subsp. elatius) were crossed with each other in both directions and also artificially pollinated with own pollen, to evaluate reproductive barriers inside Pisum sativum which may be important for pea pre-breeding. The outcome of hybrid seeds was evaluated for each combination of crosses. Reciprocal classes of F₁ hybrids were compared for pollen and seed fertility and quantitative traits including seed productivity, total biomass etc. Pollination of accession VIR320 with two accessions resulted in hybrids with drastically reduced leaves and pigmentation, resembling known cases of conflict of the nucleus and plastids. Four pairs of reciprocal F₁ hybrids showed strong differences in male and female fertility, supposedly a milder manifestation of cytonuclear conflict. Male and female fertility of hybrids correlated with each other, the former appearing more convenient for evaluating genetic disturbance of gametogenesis. Only two hybrid classes showed fully fertile pollen. Some of the accessions studied have been previously reported to differ in a reciprocal translocation and their hybrids were expected to have half-sterile pollen. However accession VIR320 seems to ‘bridge’ both karyological classes showing relatively high pollen fertility in some crosses with representatives of both of them, the reasons for which is discussed. Generally, strong and usually asymmetric cases of incompatibility manifested in the drop of fertility were revealed and their pattern did not correlate with phylogenetic relatedness of the accessions. Hence, wild representatives of P. sativum can hardly be subdivided into natural biological taxa below species rank and their involvement in pea pre-breeding can be complicated by unexpected crossing barriers.

代表野豌豆（发散谱系五个国家加入豌豆亚种。elatius）在两个方向上相互交叉，也人为地自己的花粉授粉，以评估内生殖障碍豌豆这可能是豌豆预育种重要。对每种杂交组合评估杂交种子的结果。比较了互作的F ₁杂交种的花粉和种子的受精性以及包括种子生产力，总生物量等在内的数量性状。带有两个种质的种质VIR320的授粉导致叶片显着减少和色素沉着的杂种质，类似于已知的核冲突和质体。四对倒数F ₁杂种在雄性和雌性育性上表现出强烈的差异，据认为是细胞核冲突的较轻表现。杂种的雄性和雌性相互关联，前者似乎更便于评估配子发生的遗传障碍。只有两个杂种类别显示出完全可育的花粉。先前已报道某些被研究的种质在互易易位方面存在差异，并且预期它们的杂种具有半不育花粉。但是，登录号VIR320似乎“桥接”了这两个类别的花粉育性，与它们的代表在某些杂交中显示出较高的花粉育性，并对其原因进行了讨论。一般来说，揭示了在生育力下降中表现出的强烈且通常不对称的不相容病例，并且它们的模式与种质的系统发育相关性不相关。因此，狂野的代表不能将P. sativum细分为低于物种等级的天然生物分类群，并且由于意外的穿越障碍，它们在豌豆预育中的参与也可能变得复杂。