Aedes aegypti is of utmost public health concern transmitting various diseases of human health concern. Employment of chemical-based control interventions has induced immunity in mosquitoes, harmed environment, and adversely affected human health and non-targets diverting the research focus on alternate measures. Current study investigates the efficacy of an Insect Growth Regulator, lufenuron, against early fourth instars of Ae. aegypti. The larvae exposed to lufenuron for 24 h were assessed for the effects on the development and adult emergence. The impact of sub-lethal and median-lethal dose of lufenuron was determined on the nutrients and detoxification enzymes of Ae. aegypti. The larvae exposed to lufenuron showed reduced adult emergence exhibiting the respective IE₃₀ and IE₅₀ as 0.13 μg/L and 0.96 μg/L. Larval treatment with IE₃₀ and IE₅₀ lufenuron reduced the carbohydrate and lipid content in Ae. aegypti. However, the protein levels in the larvae decreased only on exposure to IE₃₀ lufenuron while increased with IE₅₀ lufenuron. Besides, IE₃₀ and IE₅₀ lufenuron treatment elevated α-esterases (1.05-fold; 1.15-fold); β-esterases (1.29-fold;1.62-fold), and Glutathione-S-transferases (1.19-fold; 3.1-fold) expression in the Ae. aegypti larvae. The % acetylcholinesterase inhibition also reduced by 3.75-fold and 2.07-fold, correspondingly, while the cytochrome P450 monooxygenase expression rose (1.15-fold) only with IE₅₀ dosage of lufenuron. It is suggested that lufenuron stress probably ampliflied the catabolism of nutrients and expression of metabolic detoxifying enzymes in Ae. aegypti larvae in order to meet higher energy requirements and counteract the adverse effects of lufenuron. This is the first ever report unravelling the effect of lufenuron on the biochemical parameters of Ae. aegypti larvae.

埃及伊蚊最重要的公共卫生问题是传播人类健康问题的各种疾病。采用基于化学物质的控制干预措施已诱使蚊子产生免疫力，破坏了环境，对人类健康产生了不利影响，非目标人群将研究重点转移到了替代措施上。目前的研究调查了昆虫生长调节剂lufenuron对Ae早期四龄幼虫的功效。埃及 评估暴露于氟芬隆24 h的幼虫对发育和成年出苗的影响。确定了氟苯磺隆的半致死剂量和半致死剂量对Ae的营养和解毒酶的影响。埃及 暴露于氟芬隆的幼虫显示出减少的成人出苗率，并显示出相应的IE₃₀和IE _50分别为0.13μg/ L和0.96μg/ L。用IE ₃₀和IE ₅₀氟苯脲治疗幼虫可减少Ae中的碳水化合物和脂质含量。埃及 但是，幼虫中的蛋白质水平仅在暴露于IE ₃₀氟芬隆时降低，而在IE ₅₀氟芬隆时升高。此外，IE ₃₀和IE ₅₀氟苯脲治疗可使α-酯酶升高（1.05倍； 1.15倍）。Ae中的β-酯酶（1.29倍； 1.62倍）和谷胱甘肽S-转移酶（1.19倍； 3.1倍）表达。埃及幼虫。乙酰胆碱酯酶的抑制率也分别降低了3.75倍和2.07倍，而仅使用IE ₅₀剂量的氟苯磺隆，细胞色素P450单加氧酶的表达上升（1.15倍）。提示氟苯脲酮胁迫可能会放大Ae中的营养物质分解代谢和代谢解毒酶的表达。埃及eg幼虫，以满足更高的能量需求并抵消氟苯脲的不良影响。这是有史以来第一份揭示氟苯脲对Ae生化参数影响的报道。埃及伊蚊幼虫。