Abstract—

The proteomic composition of a biological sample serves as the most important feature of a biological object, and it allows discriminating normal and pathological conditions. Targeted mass spectrometric analysis, particularly, multiple reaction monitoring (MRM) using synthetic stable isotope-labeled internal standards (SIS), is the main alternative to the ELISA method for analysis of diagnostically significant proteins. Based on the MRM results, a prototype test system has been developed; it employs the targeted mass spectrometric method for multiplex, quantitative analysis of FDA-approved proteins in whole plasma. Using this approach, it was possible to measure the content of 42 proteins in 31 samples in a concentration range spanning five orders of magnitude. The interindividual variability for 30 of the 42 registered proteins was less than 40%. The largest scatter was observed for haptoglobin (68%), immunoglobulin heavy constant delta IGHD (90%), angiotensin (72%), sex hormone-binding globulin SHBG (100%), and lipoprotein-(a) (136%). The obtained results on the concentration of proteins correlate with published data (Hortin et al., Clinical Chemistry, 2008, vol. 54, 1608) with R² = 0.84. The developed prototype test system based on targeted mass spectrometric analysis of proteins can be considered as an alternative to methods using monoclonal antibodies.

中文翻译：

---

FDA批准的健康志愿者血浆中蛋白质的质谱MRM分析

摘要—

生物样品的蛋白质组学组成是生物对象的最重要特征，它可以区分正常和病理状况。靶向质谱分析，特别是使用合成的稳定同位素标记的内标（SIS）进行的多反应监测（MRM），是用于分析具有重要诊断意义的蛋白质的ELISA方法的主要替代方法。根据MRM结果，开发了一个原型测试系统。它采用靶向质谱法对整个血浆中FDA批准的蛋白质进行多重定量分析。使用这种方法，有可能在31个样品中测量5个数量级的浓度范围内42种蛋白质的含量。42种注册蛋白中有30种的个体间差异小于40％。观察到最大的散射是触珠蛋白（68％），免疫球蛋白重常数IGHD（90％），血管紧张素（72％），性激素结合球蛋白SHBG（100％）和脂蛋白-（a）（136％）。所获得的蛋白质浓度结果与已发表的数据相关（Hortin等，临床化学，2008年，第1期。54、1608），其中R ² = 0.84。基于蛋白质的目标质谱分析开发的原型测试系统可以被视为使用单克隆抗体的方法的替代方法。