Abstract—

The study aimed to investigate tissue-specific gene expression of ABCA1 and ABCG1, encoding cholesterol transporters, as well as PPARG, LXRβ (NR1H2), and RORA, encoding the most important transcriptional regulators of lipid metabolism, in subcutaneous and visceral adipose tissue (SAT and VAT) in women with metabolic syndrome. It was shown that the ABCG1 mRNA SAT/VAT ratio decreases with age and correlates with the development of metabolic syndrome. After age adjustment, women have reduced chances of metabolic syndrome development when ABCG1 gene expression in SAT is higher relative to VAT than women with VAT ABCG1 gene expression higher or comparable to SAT: OR = 0.15 (95% CI 0.03–0.76), p = 0.023. The ABCA1 mRNA SAT/VAT ratio positively correlated with HDL cholesterol levels (after age adjustment β = 0.350, p = 0.046), therefore individuals with higher ABCA1 mRNA level in SAT relative to VAT had elevated HDL levels. The ABCA1 mRNA level in SAT was decreased in smokers (p = 0.001). There was a negative correlation between the PPARG mRNA level in SAT with body mass index and waist circumference in the general sample (β = –0.602, p = 0.003 and β = –0.642, p = 0.001, respectively, after age adjustment). A decrease of the PPARG mRNA SAT/VAT ratio was associated with elevated plasma insulin level and the insulin resistance index HOMA-IR (β = –0.819, p = 0.004 and β = –1.053, p = 0.008, respectively, after age adjustment). Thus, the study has shown that the ratio of ABCA1, ABCG1, and PPARG genes expression in different types of adipose tissue (SAT/VAT) could be a significant factor that predicts the development of atherogenic dyslipidemia, metabolic syndrome, and insulin resistance in obesity.

中文翻译：

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代谢综合征女性皮下和内脏脂肪组织中编码ABCA1和ABCG1转运蛋白及PPARγ，LXRβ和RORα转录因子的基因的表达

摘要-

该研究旨在调查皮下和内脏脂肪组织中编码胆固醇转运蛋白的ABCA1和ABCG1以及编码脂质代谢最重要的转录调节因子的PPARG，LXRβ（NR1H2）和RORA的组织特异性基因表达（代谢综合征女性的SAT和VAT）。结果表明，ABCG1 mRNA SAT / VAT比值随着年龄的增长而降低，并且与代谢综合征的发生有关。调整年龄后，当SAT中的ABCG1基因表达相对于增值税要高于具有增值税ABCG1的女性时，女性代谢综合征发生的机会就会减少基因表达更高或与SAT相当：OR = 0.15（95％CI 0.03–0.76），p = 0.023。的ABCA1 mRNA的SAT / VAT比与HDL胆固醇水平（年龄调整β= 0.350，后正相关p = 0.046），因此具有较高的个体ABCA1在相对于VAT SAT mRNA水平具有升高HDL水平。的ABCA1在SAT mRNA水平在吸烟者（降低p = 0.001）。在一般样本中，SAT中的PPARG mRNA水平与体重指数和腰围呈负相关（β= –0.602，p = 0.003和β= –0.642，p调整年龄后分别为0.001）。PPARG mRNA SAT / VAT比值的降低与血浆胰岛素水平升高和胰岛素抵抗指数HOMA-IR相关（年龄调整后分别为β= –0.819，p = 0.004和β= –1.053，p = 0.008） 。因此，研究表明，不同类型的脂肪组织（SAT / VAT）中ABCA1，ABCG1和PPARG基因表达的比例可能是预测肥胖中动脉粥样硬化血脂异常，代谢综合征和胰岛素抵抗发展的重要因素。 。