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The Expression of Genes Encoding ABCA1 and ABCG1 Transporters and PPARγ, LXRβ, and RORα Transcriptional Factors in Subcutaneous and Visceral Adipose Tissue in Women with Metabolic Syndrome

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Abstract—

The study aimed to investigate tissue-specific gene expression of ABCA1 and ABCG1, encoding cholesterol transporters, as well as PPARG, LXRβ (NR1H2), and RORA, encoding the most important transcriptional regulators of lipid metabolism, in subcutaneous and visceral adipose tissue (SAT and VAT) in women with metabolic syndrome. It was shown that the ABCG1 mRNA SAT/VAT ratio decreases with age and correlates with the development of metabolic syndrome. After age adjustment, women have reduced chances of metabolic syndrome development when ABCG1 gene expression in SAT is higher relative to VAT than women with VAT ABCG1 gene expression higher or comparable to SAT: OR = 0.15 (95% CI 0.03–0.76), p = 0.023. The ABCA1 mRNA SAT/VAT ratio positively correlated with HDL cholesterol levels (after age adjustment β = 0.350, p = 0.046), therefore individuals with higher ABCA1 mRNA level in SAT relative to VAT had elevated HDL levels. The ABCA1 mRNA level in SAT was decreased in smokers (p = 0.001). There was a negative correlation between the PPARG mRNA level in SAT with body mass index and waist circumference in the general sample (β = –0.602, p = 0.003 and β = –0.642, p = 0.001, respectively, after age adjustment). A decrease of the PPARG mRNA SAT/VAT ratio was associated with elevated plasma insulin level and the insulin resistance index HOMA-IR (β = –0.819, p = 0.004 and β = –1.053, p = 0.008, respectively, after age adjustment). Thus, the study has shown that the ratio of ABCA1, ABCG1, and PPARG genes expression in different types of adipose tissue (SAT/VAT) could be a significant factor that predicts the development of atherogenic dyslipidemia, metabolic syndrome, and insulin resistance in obesity.

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ACKNOWLEDGMENTS

We are grateful to the team from the Department of General Surgery with the Clinic and the Clinic of Obstetrics and Gynecology of the Pavlov First St. Petersburg State Medical University for their assistance in preparation of biomaterial.

Funding

This study was supported by the Russian Foundation for Basic Research (project No. а 20-015-00502).

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Authors and Affiliations

  1. Konstantinov St. Petersburg Nuclear Physics Institute of National Research Centre “Kurchatov Institute”, 188300, Gatchina, Russia

    A. A. Panteleeva, N. D. Razgildina, I. A. Pobozheva, K. V. Dracheva, S. N. Pchelina & V. V. Miroshnikova

  2. Pavlov First St. Petersburg State Medical University, 197022, St. Petersburg, Russia

    A. A. Panteleeva, D. L. Brovin, I. A. Pobozheva, O. A. Berkovich, E. A. Polyakova, O. D. Belyaeva, E. I. Baranova, S. N. Pchelina & V. V. Miroshnikova

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  1. A. A. Panteleeva
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  2. N. D. Razgildina
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  3. D. L. Brovin
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  8. O. D. Belyaeva
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Correspondence to V. V. Miroshnikova.

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Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This study was approved by the ethics committee of the Pavlov First St. Petersburg State Medical University. Informed consent was obtained from all individual participants involved in the study.

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Translated by M. Novikova

Abbreviations: VAT, visceral adipose tissue; HDL, high density lipoproteins; LDL, low density lipoproteins; MS, metabolic syndrome; RCT, reverse cholesterol transport; SAT, subcutaneous adipose tissue; CL, cholesterol; HOMA-IR, insulin resistance index.

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Panteleeva, A.A., Razgildina, N.D., Brovin, D.L. et al. The Expression of Genes Encoding ABCA1 and ABCG1 Transporters and PPARγ, LXRβ, and RORα Transcriptional Factors in Subcutaneous and Visceral Adipose Tissue in Women with Metabolic Syndrome. Mol Biol 55, 56–65 (2021). https://doi.org/10.1134/S0026893321010131

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