Background

Seasonal flu is an infectious disease of the respiratory tract caused by influenza viruses. The development of anti-influenza drugs has significantly reduced the threat from the influenza virus; however, frequent mutations of this negative RNA virus result in antiviral-resistant strains, and constantly intimidate the human race. Thus, identifying novel therapeutic targets for the prevention and treatment of influenza virus infections is critical.

Objective

We aimed to determine whether the kinesin superfamily protein 11 (KIF11) inhibitors, monastrol and K858, inhibit viral cytopathogenesis and influenza A virus (IAV) replication.

Result

When MDCK or HEK293 cells were treated with monastrol and K858 that did not induce significant cytotoxicity, IAV-induced cytopathic effect was attenuated significantly. Furthermore, these inhibitors effectively suppressed the production of viral RNA, proteins, and infectious viral particles.

Conclusion

Inhibition of KIF11 activity effectively attenuates virus-mediated cytopathic effect and suppresses viral replication. Hence, KIF11 is a potential therapeutic target against the influenza virus.

中文翻译：

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KIF11抑制可减少甲型流感病毒的细胞发病机理和复制

背景

季节性流感是由流感病毒引起的呼吸道传染病。抗流感药物的开发大大减少了流感病毒的威胁；但是，这种阴性RNA病毒的频繁突变会导致产生抗病毒株，并不断威胁着人类。因此，确定用于预防和治疗流感病毒感染的新治疗靶标是至关重要的。

客观的

我们旨在确定是否驱动蛋白超家族蛋白11（KIF11）抑制剂monastrol和K858抑制病毒的细胞致病作用和甲型流感病毒（IAV）复制。

结果

当用未诱导明显细胞毒性的monastrol和K858处理MDCK或HEK293细胞时，IAV诱导的细胞病变作用显着减弱。此外，这些抑制剂可有效抑制病毒RNA，蛋白质和感染性病毒颗粒的产生。

结论

抑制KIF11活性可有效减弱病毒介导的细胞病变作用并抑制病毒复制。因此，KIF11是针对流感病毒的潜在治疗靶标。