Abstract
Background
Seasonal flu is an infectious disease of the respiratory tract caused by influenza viruses. The development of anti-influenza drugs has significantly reduced the threat from the influenza virus; however, frequent mutations of this negative RNA virus result in antiviral-resistant strains, and constantly intimidate the human race. Thus, identifying novel therapeutic targets for the prevention and treatment of influenza virus infections is critical.
Objective
We aimed to determine whether the kinesin superfamily protein 11 (KIF11) inhibitors, monastrol and K858, inhibit viral cytopathogenesis and influenza A virus (IAV) replication.
Result
When MDCK or HEK293 cells were treated with monastrol and K858 that did not induce significant cytotoxicity, IAV-induced cytopathic effect was attenuated significantly. Furthermore, these inhibitors effectively suppressed the production of viral RNA, proteins, and infectious viral particles.
Conclusion
Inhibition of KIF11 activity effectively attenuates virus-mediated cytopathic effect and suppresses viral replication. Hence, KIF11 is a potential therapeutic target against the influenza virus.
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Acknowledgements
This research was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIT) (no. 2017M3A9G6068245, NRF-2018R1A5A1025077, and 2020R1F1A1069924).
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Conceptualization, Y-JS; Methodology and investigation, J-HK and D-IK; Data analysis, J-HK, D-IK, E-HK; Statistical analysis, J-HK and D-IK; Writing—original draft, J-HK, D-IK, E-HK, and Y-JS.
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Kim, DI., Kang, JH., Kim, EH. et al. KIF11 inhibition decreases cytopathogenesis and replication of influenza A virus. Mol. Cell. Toxicol. 17, 201–212 (2021). https://doi.org/10.1007/s13273-021-00126-9
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DOI: https://doi.org/10.1007/s13273-021-00126-9