Abstract

Klebsiella pneumoniae causes a variety of human infections including pneumonia. Herein, 3 lytic bacteriophages specific for K. pneumoniae designated ΦKpnM-vB1, ΦKpnP-vB2 and ΦKpnM-vB3 were isolated and characterized. Transmission electron microscopy (TEM) analysis revealed that both ΦKpnM-vB1 and ΦKpnM-vB3 belong to family Myoviridae, while ΦKpnP-vB2 is a member of family Podoviridae. The one-step growth curve showed that ΦKpnM-vB1, ΦKpnP-vB2 and ΦKpnM-vB3 exhibited latent period of 10 min. The average burst sizes were 100, 150 and 120 PFU/cell, respectively. Isolated phages showed high thermal and pH stability. The genomic analysis indicated that ΦKpnM-vB1, ΦKpnP-vB2 and ΦKpnM-vB3 contain dsDNA genome with estimated sizes of 55, 40 and 50 Kbp, respectively. Isolated phages had lytic activity on K. pneumoniae and Escherichia coli strains. Isolated phages were highly efficient in reduction of Klebsiella biofilm suggesting their use to control biofilm formation caused by this pathogen. Isolated ΦKpnM-vB1, ΦKpnP-vB2 and ΦKpnM-vB3 are proposed to be suitable candidates for phage therapy applications. These phages offer an effective solution for treatment of infections caused by these drug-resistant bacteria.

中文翻译：

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靶向多药耐药的肺炎克雷伯菌肺炎的多价噬菌体的表征与增强的抗生物膜活性。

摘要

肺炎克雷伯菌引起多种人类感染，包括肺炎。在此，分离并鉴定了3种特异于肺炎克雷伯氏菌的裂解噬菌体，分别称为ΦKpnM-vB1，ΦKpnP-vB2和ΦKpnM-vB3。透射电镜（TEM）分析表明ΦKpnM-vB1和ΦKpnM-vB3都属于肌病毒科，而ΦKpnP-vB2属于Podoviridae科。。一步生长曲线表明，ΦKpnM-vB1，ΦKpnP-vB2和ΦKpnM-vB3的潜伏期为10分钟。平均突发大小分别为100、150和120 PFU /单元。分离的噬菌体显示出高的热稳定性和pH稳定性。基因组分析表明，ΦKpnM-vB1，ΦKpnP-vB2和ΦKpnM-vB3包含dsDNA基因组，其估计大小分别为55、40和50 Kbp。分离的噬菌体对肺炎克雷伯菌和大肠杆菌菌株具有裂解活性。分离的噬菌体在减少克雷伯菌中非常有效生物膜表明它们可用于控制由该病原体引起的生物膜形成。提出分离的ΦKpnM-vB1，ΦKpnP-vB2和ΦKpnM-vB3是噬菌体治疗应用的合适候选物。这些噬菌体为治疗由这些抗药性细菌引起的感染提供了有效的解决方案。