A 45-day feeding trial was conducted to evaluate the effect of dietary jamun tree leaf (JL) on the antioxidant defence system-based disease resistance in juveniles of Trachinotus blochii. The juveniles of snubnose pompano were distributed into four treatment groups in triplicates. Each treatment was fed with a diet containing either 0 (0JL), 0.5 (0.5JL), 1 (1JL) and 1.5% JL (1.5JL) in the feed. After feeding trial, the fishes were experimentally infected with Vibrio parahaemolyticus. The activities of oxidative stress enzymes such as superoxide dismutase and catalase were found to be increasing with increasing level of dietary JL incorporation, and the lower value was witnessed in control group in pre- and post-challenge. After challenge, the alanine and aspartate aminotransferase activities in all the treatments were significantly increased (P < 0.05) than the pre-challenge condition and exhibited reverse trend with the antioxidant enzymes. The alkaline and acid phosphatase activities were found higher in 1.5JL group and showed significant difference (P < 0.05) among the treatments. The respiratory burst activity and liver glycogen content showed an increasing trend as the level of inclusion of JL increased in the diet. The acetylcholinesterase activity was significantly plunged (P < 0.05) after experimental infection, and JL diet fed groups showed better activity. After experimental infection with V. parahaemolyticus, the highest relative percentage of survival was observed in 1JL and 1.5JL groups. Hence, dietary supplementation of jamun tree leaf at the level of 1% is adequate to reduce the oxidative stress and improved the innate immune status through antioxidant defence system.

进行了为期45天的喂养试验，以评估日粮Jamun树叶（JL）对Trachnotus blochii幼鱼基于抗氧化防御系统的疾病抵抗力的影响。sn鱼的少年一式三份地分为四个治疗组。每种处理均饲喂饲料中含有0（0JL），0.5（0.5JL），1（1JL）和1.5％JL（1.5JL）的饮食。喂食试验后，将鱼用副溶血弧菌进行实验感染。发现氧化应激酶（如超氧化物歧化酶和过氧化氢酶）的活性随日粮JL掺入量的增加而增加，而在挑战前后，对照组的值较低。攻击后，与攻击前相比，所有处理中的丙氨酸和天冬氨酸转氨酶活性均显着增加（P <0.05），并与抗氧化酶呈现相反的趋势。1.5JL组的碱性和酸性磷酸酶活性较高，差异有统计学意义（P<0.05）。随着饮食中JL含量的增加，呼吸爆发活性和肝糖原含量呈增加趋势。实验性感染后，乙酰胆碱酯酶活性显着下降（P <0.05），而JL日粮喂养组表现出更好的活性。实验性副溶血性弧菌感染后，在1JL和1.5JL组中观察到最高的相对存活百分比。因此，在膳食中添加1％的Jamun树叶足以通过抗氧化防御系统降低氧化应激并改善先天免疫状态。