Abstract
A 45-day feeding trial was conducted to evaluate the effect of dietary jamun tree leaf (JL) on the antioxidant defence system-based disease resistance in juveniles of Trachinotus blochii. The juveniles of snubnose pompano were distributed into four treatment groups in triplicates. Each treatment was fed with a diet containing either 0 (0JL), 0.5 (0.5JL), 1 (1JL) and 1.5% JL (1.5JL) in the feed. After feeding trial, the fishes were experimentally infected with Vibrio parahaemolyticus. The activities of oxidative stress enzymes such as superoxide dismutase and catalase were found to be increasing with increasing level of dietary JL incorporation, and the lower value was witnessed in control group in pre- and post-challenge. After challenge, the alanine and aspartate aminotransferase activities in all the treatments were significantly increased (P < 0.05) than the pre-challenge condition and exhibited reverse trend with the antioxidant enzymes. The alkaline and acid phosphatase activities were found higher in 1.5JL group and showed significant difference (P < 0.05) among the treatments. The respiratory burst activity and liver glycogen content showed an increasing trend as the level of inclusion of JL increased in the diet. The acetylcholinesterase activity was significantly plunged (P < 0.05) after experimental infection, and JL diet fed groups showed better activity. After experimental infection with V. parahaemolyticus, the highest relative percentage of survival was observed in 1JL and 1.5JL groups. Hence, dietary supplementation of jamun tree leaf at the level of 1% is adequate to reduce the oxidative stress and improved the innate immune status through antioxidant defence system.
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The authors are thankful to Director, ICAR-CMFRI, Kochi, for their constant support to carry out the research work. The authors are grateful to Dr. L. Ranjith and Dr. C. Kalidas for their support in language and grammar editing. The authors are also indebted to Dr. P. Sayooj and Mr. P. Vishnu for rendering their untiring support in the wet laboratory operation throughout the research work.
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This project was supported by funding from Indian Council of Agricultural research under the project with the code: CMFRI/MBTD/NTN/24 during 2017-2020.
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Dhanasekaran Linga Prabu: Conceptualization; Designed and conducted the experiment; draft preparation of MS
Sanal Ebeneezar: Associated with the laboratory analysis; Associated in draft preparation
Selvam Chandrasekar: Associated with the laboratory analysis
Mookaiah Kavitha: Correction of manuscript, statistical analysis
Pananghat Vijayagopal: Mentor; experimental designs; Review and correction of MS
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Prabu, D.L., Ebeneezar, S., Chandrasekar, S. et al. Antioxidant defence system based oxidative stress mitigation through dietary jamun tree leaf in experimentally infected snubnose pompano, Trachinotus blochii. Fish Physiol Biochem 47, 617–637 (2021). https://doi.org/10.1007/s10695-021-00935-8
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DOI: https://doi.org/10.1007/s10695-021-00935-8